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- W2014045710 abstract "In bacteria, the RecA protein plays important roles in a number of DNA recombination and repair processes, including homologous recombination, SOS induction and recombinational DNA repair. We have explored the idea that the Escherichia coli RecA protein’s functions could be controlled by small molecules. We investigated the 2:1 complex of zinc(II) with 1,4-dithio-l-threitol (l-DTT) that inhibits the E. coli rho transcription terminator, which is a hexameric ATP motor protein and is structurally homologous to RecA. We found that both the complex and ZnCl2 inhibit the single-stranded DNA-dependent ATPase activity of RecA at sub-millimolar concentrations. Investigation of a variety of metal dications (0.4 mM final concentration) determined that zinc(II), copper(II) and mercury(II) all induce the precipitation of RecA, while the dichloride salts of calcium, manganese, barium, cobalt, and nickel do not. The inhibition of RecA activity by Zn(II), Cu(II) and Hg(II) results from the metal-dependent initiation of RecA aggregation. These observations may have implications for the design of biophysical experiments requiring solid-phase RecA protein, for a more complete understanding of metal toxicities, and for the design of metal-chelate inhibitors of prokaryotic DNA repair." @default.
- W2014045710 created "2016-06-24" @default.
- W2014045710 creator A5050975138 @default.
- W2014045710 creator A5053842268 @default.
- W2014045710 date "2004-11-01" @default.
- W2014045710 modified "2023-10-18" @default.
- W2014045710 title "Inhibition of the Escherichia coli RecA protein: zinc(II), copper(II) and mercury(II) trap RecA as inactive aggregates" @default.
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- W2014045710 cites W1531956201 @default.
- W2014045710 cites W1546027199 @default.
- W2014045710 cites W1570361288 @default.
- W2014045710 cites W1575122049 @default.
- W2014045710 cites W1580743957 @default.
- W2014045710 cites W1583942422 @default.
- W2014045710 cites W1590140535 @default.
- W2014045710 cites W1595808248 @default.
- W2014045710 cites W1597599526 @default.
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- W2014045710 cites W1656081390 @default.
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- W2014045710 cites W1984786939 @default.
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- W2014045710 doi "https://doi.org/10.1016/j.jinorgbio.2004.08.018" @default.
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