FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014050671> ?p ?o ?g. }

• W2014050671 endingPage "32701" @default.
• W2014050671 startingPage "32695" @default.
• W2014050671 abstract "Transcription by RNA polymerase III (pol III) in yeast requires the assembly of an initiation complex comprising the TATA-binding protein (TBP), a 90-kDa polypeptide (TFIIIB90), and a 70-kDa polypeptide (TFIIIB70). TFIIIB70 interacts with TBP, a unique pol III subunit, C34, and the 131-kDa subunit of the pol III-specific complex, TFIIIC. TFIIIB70 was expressed in Escherichia coli and purified to homogeneity. The specific transcription activity of rTFIIIB70 is 22-58% that of the native yeast and in vitro synthesized factor. However, only a small fraction (0.07-0.32%) of the TFIIIB70 from these sources results in the synthesis of full-length RNA. The data suggest that TFIIIB70 function may be limited by an unfavorable recruitment equilibrium into the preinitiation complex. Quantitative DNase I “footprint” titrations of yeast TBP to the adenovirus major late promoter were conducted at a series of constant TFIIIB70 concentrations. A value of −0.7 ± 0.2 kcal/mol was determined for the cooperative free energy of formation of the TBP·TFIIIB70·DNA complex at concentrations of TFIIIB70 sufficient to partition all of the binding cooperativity to the TBP binding isotherm. A Kd of 44 ± 23 nM characterizes the TFIIIB70 concentration dependence of the TBP·TFIIIB70 cooperativity. The relationship δlog K/δlog (TFIIIB70) is consistent with the linkage of a single molecule of TFIIIB70 with the TBP-promoter binding reaction. Transcription by RNA polymerase III (pol III) in yeast requires the assembly of an initiation complex comprising the TATA-binding protein (TBP), a 90-kDa polypeptide (TFIIIB90), and a 70-kDa polypeptide (TFIIIB70). TFIIIB70 interacts with TBP, a unique pol III subunit, C34, and the 131-kDa subunit of the pol III-specific complex, TFIIIC. TFIIIB70 was expressed in Escherichia coli and purified to homogeneity. The specific transcription activity of rTFIIIB70 is 22-58% that of the native yeast and in vitro synthesized factor. However, only a small fraction (0.07-0.32%) of the TFIIIB70 from these sources results in the synthesis of full-length RNA. The data suggest that TFIIIB70 function may be limited by an unfavorable recruitment equilibrium into the preinitiation complex. Quantitative DNase I “footprint” titrations of yeast TBP to the adenovirus major late promoter were conducted at a series of constant TFIIIB70 concentrations. A value of −0.7 ± 0.2 kcal/mol was determined for the cooperative free energy of formation of the TBP·TFIIIB70·DNA complex at concentrations of TFIIIB70 sufficient to partition all of the binding cooperativity to the TBP binding isotherm. A Kd of 44 ± 23 nM characterizes the TFIIIB70 concentration dependence of the TBP·TFIIIB70 cooperativity. The relationship δlog K/δlog (TFIIIB70) is consistent with the linkage of a single molecule of TFIIIB70 with the TBP-promoter binding reaction." @default.
• W2014050671 created "2016-06-24" @default.
• W2014050671 creator A5010967667 @default.
• W2014050671 creator A5017045603 @default.
• W2014050671 creator A5048335197 @default.
• W2014050671 creator A5080003680 @default.
• W2014050671 date "1996-12-01" @default.
• W2014050671 modified "2023-09-27" @default.
• W2014050671 title "Expression and Purification of the RNA Polymerase III Transcription Specificity Factor IIIB70 from Saccharomyces cerevisiae and Its Cooperative Binding with TATA-binding Protein" @default.
• W2014050671 cites W1521470590 @default.
• W2014050671 cites W1523752512 @default.
• W2014050671 cites W1527043413 @default.
• W2014050671 cites W1543166364 @default.
• W2014050671 cites W1547123197 @default.
• W2014050671 cites W1550808793 @default.
• W2014050671 cites W1576948329 @default.
• W2014050671 cites W1873199501 @default.
• W2014050671 cites W1876905084 @default.
• W2014050671 cites W1965700324 @default.
• W2014050671 cites W1966171579 @default.
• W2014050671 cites W1968764028 @default.
• W2014050671 cites W1977694232 @default.
• W2014050671 cites W1979229978 @default.
• W2014050671 cites W1989451462 @default.
• W2014050671 cites W1994706623 @default.
• W2014050671 cites W2004912100 @default.
• W2014050671 cites W2006630070 @default.
• W2014050671 cites W2012473850 @default.
• W2014050671 cites W2015020895 @default.
• W2014050671 cites W2020300023 @default.
• W2014050671 cites W2027281272 @default.
• W2014050671 cites W2032864324 @default.
• W2014050671 cites W2035797443 @default.
• W2014050671 cites W2039782503 @default.
• W2014050671 cites W2044103662 @default.
• W2014050671 cites W2045947099 @default.
• W2014050671 cites W2055207649 @default.
• W2014050671 cites W2060650180 @default.
• W2014050671 cites W2069748359 @default.
• W2014050671 cites W2073501728 @default.
• W2014050671 cites W2073515063 @default.
• W2014050671 cites W2075239951 @default.
• W2014050671 cites W2083842078 @default.
• W2014050671 cites W2087727696 @default.
• W2014050671 cites W2094860959 @default.
• W2014050671 cites W2098195130 @default.
• W2014050671 cites W2121415381 @default.
• W2014050671 cites W2137634127 @default.
• W2014050671 cites W2162283732 @default.
• W2014050671 cites W2166464288 @default.
• W2014050671 cites W230984257 @default.
• W2014050671 cites W2414811937 @default.
• W2014050671 doi "https://doi.org/10.1074/jbc.271.51.32695" @default.
• W2014050671 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8955101" @default.
• W2014050671 hasPublicationYear "1996" @default.
• W2014050671 type Work @default.
• W2014050671 sameAs 2014050671 @default.
• W2014050671 citedByCount "21" @default.
• W2014050671 countsByYear W20140506712019 @default.
• W2014050671 countsByYear W20140506712021 @default.
• W2014050671 crossrefType "journal-article" @default.
• W2014050671 hasAuthorship W2014050671A5010967667 @default.
• W2014050671 hasAuthorship W2014050671A5017045603 @default.
• W2014050671 hasAuthorship W2014050671A5048335197 @default.
• W2014050671 hasAuthorship W2014050671A5080003680 @default.
• W2014050671 hasBestOaLocation W20140506711 @default.
• W2014050671 hasConcept C101762097 @default.
• W2014050671 hasConcept C104292427 @default.
• W2014050671 hasConcept C104317684 @default.
• W2014050671 hasConcept C105208137 @default.
• W2014050671 hasConcept C107824862 @default.
• W2014050671 hasConcept C138885662 @default.
• W2014050671 hasConcept C144825837 @default.
• W2014050671 hasConcept C150194340 @default.
• W2014050671 hasConcept C153911025 @default.
• W2014050671 hasConcept C166014724 @default.
• W2014050671 hasConcept C172768829 @default.
• W2014050671 hasConcept C179926584 @default.
• W2014050671 hasConcept C187250156 @default.
• W2014050671 hasConcept C188740287 @default.
• W2014050671 hasConcept C25281209 @default.
• W2014050671 hasConcept C2776449523 @default.
• W2014050671 hasConcept C2780055153 @default.
• W2014050671 hasConcept C2909959438 @default.
• W2014050671 hasConcept C41895202 @default.
• W2014050671 hasConcept C44498088 @default.
• W2014050671 hasConcept C552990157 @default.
• W2014050671 hasConcept C55493867 @default.
• W2014050671 hasConcept C67705224 @default.
• W2014050671 hasConcept C70916841 @default.
• W2014050671 hasConcept C82381507 @default.
• W2014050671 hasConcept C86339819 @default.
• W2014050671 hasConcept C86803240 @default.
• W2014050671 hasConcept C94966510 @default.
• W2014050671 hasConceptScore W2014050671C101762097 @default.
• W2014050671 hasConceptScore W2014050671C104292427 @default.
• W2014050671 hasConceptScore W2014050671C104317684 @default.
• W2014050671 hasConceptScore W2014050671C105208137 @default.

• next