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- W2014059216 abstract "To determine whether serum supplementation influenced fatty acid content of bovine blastocysts and whether vitamin E addition to culture medium containing serum could improve development in vitro, cleaved eggs were cultured in synthetic oviduct fluid supplemented with bovine serum albumin (BSA, 0.4% w/v, fraction V) (SVBSA), fetal calf serum (FCS, 10% v/v) (SFCS) or FCS (10% v/v) plus 100 μM vitamin E (SFCS + E). Blastocyst yields were recorded and fatty acid composition was determined by gas chromatography. Day 7 blastocysts were incubated with [2-14C] pyruvate for 3 h and then fixed for cell counts. Yields of good quality blastocysts were greatest from cleaved eggs cultured in serum-free conditions (P < 0.01). In the presence of serum, supplementation with vitamin E increased both total and good quality blastocyst yields (P < 0.01). Presence of serum increased fatty acid content (mean ± SEM) of blastocysts (SVBSA v. SFCS = 57 ± 2 v. 74 ± 2 ng embryo−1; P < 0.001). In contrast, pyruvate metabolism was greater in blastocysts produced without serum (27 ± 3 v. 21 ± 3 picomoles embryo−13 h−1; P < 0.01) but, on a per cell basis, no differences were detected. Addition of vitamin E to the serum-supplemented formulation did not alter either the fatty acid content (73 ± 2 ng embryo−1) or pyruvate metabolism index (19 ± 1 pmol embryo−13 h−1) of SFCS + E blastocysts. Thus, despite lipid accumulation, supplementary vitamin E improved blastocyst yields in embryos exposed to serum." @default.
- W2014059216 created "2016-06-24" @default.
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- W2014059216 date "2003-01-01" @default.
- W2014059216 modified "2023-10-14" @default.
- W2014059216 title "Consequences of exposure to serum, with or without vitamin E supplementation, in terms of the fatty acid content and viability of bovine blastocysts produced in vitro" @default.
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- W2014059216 doi "https://doi.org/10.1071/rd03004" @default.
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