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- W2014071844 abstract "Effective vaccine development for human immunodeficiency virus type 1 (HIV-1) will require assays that ascertain the capacity of vaccine immunogens to elicit neutralizing antibodies (NAb) to diverse HIV-1 strains. To facilitate NAb assessment in peripheral blood mononuclear cell (PBMC)-based assays, we developed an assay-adaptable platform based on a Renilla luciferase (LucR) expressing HIV-1 proviral backbone. LucR was inserted into pNL4-3 DNA, preserving all viral open reading frames. The proviral genome was engineered to facilitate expression of diverse HIV-1 env sequences, allowing analysis in an isogenic background. The resulting Env-IMC-LucR viruses are infectious, and LucR is stably expressed over multiple replications in PBMC. HIV-1 neutralization, targeting TZM-bl cells, was highly correlative comparing virus (LucR) and cell (firefly luciferase) readouts. In PBMC, NAb activity can be analyzed either within a single or multiple cycles of replication. These results represent advancement toward a standardizable PBMC-based neutralization assay for assessing HIV-1 vaccine immunogen efficacy." @default.
- W2014071844 created "2016-06-24" @default.
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- W2014071844 date "2010-12-01" @default.
- W2014071844 modified "2023-09-27" @default.
- W2014071844 title "Replication competent molecular clones of HIV-1 expressing Renilla luciferase facilitate the analysis of antibody inhibition in PBMC" @default.
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- W2014071844 doi "https://doi.org/10.1016/j.virol.2010.08.028" @default.
- W2014071844 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2993081" @default.
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- W2014071844 hasPublicationYear "2010" @default.
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