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- W2014074382 abstract "Despite the combination of light-microscopic immunocytochemistry, histochemical mRNA detection techniques and protein reporter systems, progress in identifying the protein composition of neuronal versus glial gap junctions, determination of the differential localization of their constituent connexin proteins in two apposing membranes and understanding human neurological diseases caused by connexin mutations has been problematic due to ambiguities introduced in the cellular and subcellular assignment of connexins. Misassignments occurred primarily because membranes and their constituent proteins are below the limit of resolution of light microscopic imaging techniques. Currently, only serial thin-section transmission electron microscopy and freeze-fracture replica immunogold labeling have sufficient resolution to assign connexin proteins to either or both sides of gap junction plaques. However, freeze-fracture replica immunogold labeling has been limited because conventional freeze fracturing allows retrieval of only one of the two membrane fracture faces within a gap junction, making it difficult to identify connexin coupling partners in hemiplaques removed by fracturing. We now summarize progress in ascertaining the connexin composition of two coupled hemiplaques using matched double-replicas that are labeled simultaneously for multiple connexins. This approach allows unambiguous identification of connexins and determination of the membrane sidedness and the identities of connexin coupling partners in homotypic and heterotypic gap junctions of vertebrate neurons." @default.
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- W2014074382 date "2012-06-01" @default.
- W2014074382 modified "2023-09-27" @default.
- W2014074382 title "Connexin Composition in Apposed Gap Junction Hemiplaques Revealed by Matched Double-Replica Freeze-Fracture Replica Immunogold Labeling" @default.
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- W2014074382 doi "https://doi.org/10.1007/s00232-012-9454-2" @default.
- W2014074382 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3401501" @default.
- W2014074382 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22760604" @default.
- W2014074382 hasPublicationYear "2012" @default.
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