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- W2014085264 abstract "Dibutyltin-3-hydroxyflavone bromide, Bu2Sn(of), is a fluorescent probe inhibitor of mitochondrial F1F0ATPase which reacts with and titrates a component of F0 with marked fluorescence enhancement and reacts similarly with chloroplast CF1CF0 and V-ATPases. Its use to monitor the interactions of other F0 inhibitors (venturicidin, oligomycin, DCCD) with F1F0ATPase, both membrane-bound and purified by solubilization is described. Trialkyltins (Bu3SnCl) back-titrate all Bu2Sn(of) interaction sites; whereas the macrolide inhibitor venturicidin backtitrates 60±5% and oligomycin only 30±3% of Bu2Sn(of) interaction sites. Bafilomycin, the macrolide inhibitor of V-ATPases, is inactive in this assay. DCCD acts in a different fashion from the other inhibitors. Current and potential applications of this fluorescent probe in mitochondrial bioenergetics and biogenesis are discussed." @default.
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- W2014085264 date "1993-10-01" @default.
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- W2014085264 title "The F1F0-ATPase binding site of dibutyltin-3-hydroxyflavone: Interactions with venturicidin, oligomycin and DCCD" @default.
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- W2014085264 doi "https://doi.org/10.1002/aoc.590070607" @default.
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