Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014086919> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W2014086919 endingPage "414a" @default.
- W2014086919 startingPage "414a" @default.
- W2014086919 abstract "Measuring the binding curve and stoichiometry of protein complexes in living cells is a prerequisite for quantitative modeling of cellular processes. Dual-color fluorescence fluctuation spectroscopy provides a general framework for detecting protein interactions. However, quantitative characterization of protein hetero-interactions remains a difficult task. To address this challenge we introduce hetero-species partition (HSP) analysis for measuring protein hetero-interactions of the type D + nA -> DAn. HSP directly identifies the hetero-interacting species from the sample mixture and determines the binding curve and stoichiometry in the cellular environment. The method is applied to measure the ligand-dependent binding curve of the nuclear receptor retinoic X receptor to the coactivator transcription intermediate factor 2. The binding stoichiometry of this protein system has not been directly measured yet. A previous study using protein fragments observed a higher binding stoichiometry than biologically expected. We address this difference in stoichiometry by measuring the binding curves of the full-length proteins in living cells. This study provides proof-of-principle experiments that illustrate the potential of HSP as a general and robust analysis tool for the quantitative characterization of protein hetero-interactions in living cells." @default.
- W2014086919 created "2016-06-24" @default.
- W2014086919 creator A5037458498 @default.
- W2014086919 creator A5057734727 @default.
- W2014086919 creator A5090868384 @default.
- W2014086919 date "2010-01-01" @default.
- W2014086919 modified "2023-09-28" @default.
- W2014086919 title "Observing Nuclear Receptor / Coactivator Interactions in Live Cells by Hetero-Species Partition Analysis" @default.
- W2014086919 doi "https://doi.org/10.1016/j.bpj.2009.12.2234" @default.
- W2014086919 hasPublicationYear "2010" @default.
- W2014086919 type Work @default.
- W2014086919 sameAs 2014086919 @default.
- W2014086919 citedByCount "0" @default.
- W2014086919 crossrefType "journal-article" @default.
- W2014086919 hasAuthorship W2014086919A5037458498 @default.
- W2014086919 hasAuthorship W2014086919A5057734727 @default.
- W2014086919 hasAuthorship W2014086919A5090868384 @default.
- W2014086919 hasBestOaLocation W20140869191 @default.
- W2014086919 hasConcept C104317684 @default.
- W2014086919 hasConcept C114614502 @default.
- W2014086919 hasConcept C11804247 @default.
- W2014086919 hasConcept C12554922 @default.
- W2014086919 hasConcept C144082473 @default.
- W2014086919 hasConcept C147789679 @default.
- W2014086919 hasConcept C185592680 @default.
- W2014086919 hasConcept C33923547 @default.
- W2014086919 hasConcept C42812 @default.
- W2014086919 hasConcept C55493867 @default.
- W2014086919 hasConcept C77445288 @default.
- W2014086919 hasConcept C86339819 @default.
- W2014086919 hasConcept C86803240 @default.
- W2014086919 hasConceptScore W2014086919C104317684 @default.
- W2014086919 hasConceptScore W2014086919C114614502 @default.
- W2014086919 hasConceptScore W2014086919C11804247 @default.
- W2014086919 hasConceptScore W2014086919C12554922 @default.
- W2014086919 hasConceptScore W2014086919C144082473 @default.
- W2014086919 hasConceptScore W2014086919C147789679 @default.
- W2014086919 hasConceptScore W2014086919C185592680 @default.
- W2014086919 hasConceptScore W2014086919C33923547 @default.
- W2014086919 hasConceptScore W2014086919C42812 @default.
- W2014086919 hasConceptScore W2014086919C55493867 @default.
- W2014086919 hasConceptScore W2014086919C77445288 @default.
- W2014086919 hasConceptScore W2014086919C86339819 @default.
- W2014086919 hasConceptScore W2014086919C86803240 @default.
- W2014086919 hasIssue "3" @default.
- W2014086919 hasLocation W20140869191 @default.
- W2014086919 hasOpenAccess W2014086919 @default.
- W2014086919 hasPrimaryLocation W20140869191 @default.
- W2014086919 hasRelatedWork W2012799867 @default.
- W2014086919 hasRelatedWork W2057841153 @default.
- W2014086919 hasRelatedWork W2067931052 @default.
- W2014086919 hasRelatedWork W2110646266 @default.
- W2014086919 hasRelatedWork W2138469499 @default.
- W2014086919 hasRelatedWork W2318149005 @default.
- W2014086919 hasRelatedWork W2404304713 @default.
- W2014086919 hasRelatedWork W2606720811 @default.
- W2014086919 hasRelatedWork W4234183479 @default.
- W2014086919 hasRelatedWork W4254747857 @default.
- W2014086919 hasVolume "98" @default.
- W2014086919 isParatext "false" @default.
- W2014086919 isRetracted "false" @default.
- W2014086919 magId "2014086919" @default.
- W2014086919 workType "article" @default.