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- W2014106710 abstract "To prevent potential errors in protein synthesis, some aminoacyl-transfer RNA (tRNA) synthetases have evolved editing mechanisms to hydrolyze misactivated amino acids (pre-transfer editing) or misacylated tRNAs (post-transfer editing). Class Ia leucyl-tRNA synthetase (LeuRS) may misactivate various natural and non-protein amino acids and then mischarge tRNA Leu . It is known that the fidelity of prokaryotic LeuRS depends on multiple editing pathways to clear the incorrect intermediates and products in the every step of aminoacylation reaction. Here, we obtained human cytoplasmic LeuRS (hcLeuRS) and tRNA Leu (hctRNA Leu ) with high activity from Escherichia coli overproducing strains to study the synthetic and editing properties of the enzyme. We revealed that hcLeuRS could adjust its editing strategy against different non-cognate amino acids. HcLeuRS edits norvaline predominantly by post-transfer editing; however, it uses mainly pre-transfer editing to edit α-amino butyrate, although both amino acids can be charged to tRNA Leu . Post-transfer editing as a final checkpoint of the reaction was very important to prevent mis-incorporation in vitro . These results provide insight into the modular editing pathways created to prevent genetic code ambiguity by evolution." @default.
- W2014106710 created "2016-06-24" @default.
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- W2014106710 date "2010-08-30" @default.
- W2014106710 modified "2023-10-14" @default.
- W2014106710 title "Modular pathways for editing non-cognate amino acids by human cytoplasmic leucyl-tRNA synthetase" @default.
- W2014106710 cites W1027635430 @default.
- W2014106710 cites W1480195325 @default.
- W2014106710 cites W1495386551 @default.
- W2014106710 cites W1581318482 @default.
- W2014106710 cites W1857572381 @default.
- W2014106710 cites W1923250545 @default.
- W2014106710 cites W1965294448 @default.
- W2014106710 cites W1970318558 @default.
- W2014106710 cites W1970695197 @default.
- W2014106710 cites W1971604056 @default.
- W2014106710 cites W1972780119 @default.
- W2014106710 cites W1979414125 @default.
- W2014106710 cites W1980382519 @default.
- W2014106710 cites W1980461282 @default.
- W2014106710 cites W1981529363 @default.
- W2014106710 cites W1981900522 @default.
- W2014106710 cites W1985888036 @default.
- W2014106710 cites W1986561802 @default.
- W2014106710 cites W1989562242 @default.
- W2014106710 cites W1991202844 @default.
- W2014106710 cites W1991324280 @default.
- W2014106710 cites W1993702783 @default.
- W2014106710 cites W2002944601 @default.
- W2014106710 cites W2003734450 @default.
- W2014106710 cites W2004416250 @default.
- W2014106710 cites W2007982521 @default.
- W2014106710 cites W2008264319 @default.
- W2014106710 cites W2009746802 @default.
- W2014106710 cites W2011857146 @default.
- W2014106710 cites W2014478039 @default.
- W2014106710 cites W2016059768 @default.
- W2014106710 cites W2016485550 @default.
- W2014106710 cites W2021463176 @default.
- W2014106710 cites W2025539681 @default.
- W2014106710 cites W2026234586 @default.
- W2014106710 cites W2036130241 @default.
- W2014106710 cites W2037825700 @default.
- W2014106710 cites W2043406399 @default.
- W2014106710 cites W2044132125 @default.
- W2014106710 cites W2045445805 @default.
- W2014106710 cites W2046129360 @default.
- W2014106710 cites W2048284469 @default.
- W2014106710 cites W2053769208 @default.
- W2014106710 cites W2053811117 @default.
- W2014106710 cites W2055679353 @default.
- W2014106710 cites W2055804317 @default.
- W2014106710 cites W2056752459 @default.
- W2014106710 cites W2057976012 @default.
- W2014106710 cites W2063309485 @default.
- W2014106710 cites W2066392481 @default.
- W2014106710 cites W2066768470 @default.
- W2014106710 cites W2074616759 @default.
- W2014106710 cites W2077855194 @default.
- W2014106710 cites W2078707325 @default.
- W2014106710 cites W2081935857 @default.
- W2014106710 cites W2082846797 @default.
- W2014106710 cites W2082957812 @default.
- W2014106710 cites W2083756379 @default.
- W2014106710 cites W2083794840 @default.
- W2014106710 cites W2088742868 @default.
- W2014106710 cites W2089143068 @default.
- W2014106710 cites W2091067056 @default.
- W2014106710 cites W2094291868 @default.
- W2014106710 cites W2120744038 @default.
- W2014106710 cites W2130033465 @default.
- W2014106710 cites W2134862369 @default.
- W2014106710 cites W2140885472 @default.
- W2014106710 cites W2150350729 @default.
- W2014106710 cites W2152895940 @default.
- W2014106710 cites W2157548025 @default.
- W2014106710 cites W2359463435 @default.
- W2014106710 doi "https://doi.org/10.1093/nar/gkq763" @default.
- W2014106710 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3017609" @default.
- W2014106710 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20805241" @default.
- W2014106710 hasPublicationYear "2010" @default.
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