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• W2014129784 abstract "The depletion of immune T cells by human immunodeficiency virus type-1 (HIV-1) infection is a major mechanism involved in the pathogenesis of AIDS. Here, we examined a possible effector function of blood monocyte-derived dendritic cells (DCs) to induce apoptosis in bystander CD4+ and CD8+ T cells. The DCs were generated by culturing monocytes in the presence of granulocyte-macrophage colony-stimulating factor and interleukin-4. The DCs exposed to HIV-1 particles were co-cultured with healthy donor-derived blood T cells at a ratio of 1:20. Analyses by percent cell mortality, staining with propidium iodide and reactivity with Annexin V revealed the induction of apoptosis in both CD4+ and CD8+ target T cells. Further, this apoptosis occurred without stimulation with mitogens when the cell cycle of target T cells shifted from G0 to G1, probably due to the mitogenic effect of the DCs. Thus, induction of apoptosis in both CD4+ and CD8+ T cells occurred via interaction with DCs adsorbed with HIV-1 particles." @default.
• W2014129784 created "2016-06-24" @default.
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• W2014129784 date "2000-02-01" @default.
• W2014129784 modified "2023-10-12" @default.
• W2014129784 title "Exposure of Normal Monocyte-Derived Dendritic Cells to Human Immunodeficiency Virus Type-1 Particles Leads to the Induction of Apoptosis in Co-Cultured CD4⁺as Well as CD8⁺T Cells" @default.
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• W2014129784 doi "https://doi.org/10.1111/j.1348-0421.2000.tb01254.x" @default.
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