FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014148312> ?p ?o ?g. }

• W2014148312 endingPage "93" @default.
• W2014148312 startingPage "82" @default.
• W2014148312 abstract "Escherichia coli DksA and GreB bind to RNA polymerase (RNAP), reaching inside the secondary channel, with similar affinities but have different cellular functions. DksA destabilizes promoter complexes whereas GreB facilitates RNA cleavage in arrested elongation complexes (ECs). Although the less abundant GreB may not interfere with DksA regulation during initiation, reports that DksA acts during elongation and termination suggest that it may exclude GreB from arrested complexes, potentially triggering genome instability. Here, we show that GreB does not compete with DksA during termination whereas DksA, even when present in several hundredfold molar excess, does not inhibit GreB-mediated cleavage of the nascent RNA. Our findings that DksA does not bind to backtracked or active ECs provide an explanation for the lack of DksA activity on most ECs that we reported previously, raising a question of what makes a transcription complex susceptible to DksA. Structural modeling suggests that i6, an insertion in the catalytic trigger loop, hinders DksA access into the channel, restricting DksA action to a subset of transcription complexes. In support of this hypothesis, we demonstrate that deletion of i6 permits DksA binding to ECs and that the distribution of DksA and i6 in bacterial genomes is strongly concordant. We hypothesize that DksA binds to transcription complexes in which i6 becomes mobile, for example, as a consequence of weakened RNAP interactions with the downstream duplex DNA." @default.
• W2014148312 created "2016-06-24" @default.
• W2014148312 creator A5012792532 @default.
• W2014148312 creator A5044773791 @default.
• W2014148312 creator A5063187030 @default.
• W2014148312 creator A5085424463 @default.
• W2014148312 date "2013-01-01" @default.
• W2014148312 modified "2023-09-25" @default.
• W2014148312 title "An Insertion in the Catalytic Trigger Loop Gates the Secondary Channel of RNA Polymerase" @default.
• W2014148312 cites W1573968512 @default.
• W2014148312 cites W1709473677 @default.
• W2014148312 cites W1967725326 @default.
• W2014148312 cites W1970103400 @default.
• W2014148312 cites W1970798296 @default.
• W2014148312 cites W1975941280 @default.
• W2014148312 cites W1979360127 @default.
• W2014148312 cites W1979932438 @default.
• W2014148312 cites W1982327953 @default.
• W2014148312 cites W1998192639 @default.
• W2014148312 cites W1999718691 @default.
• W2014148312 cites W2004971378 @default.
• W2014148312 cites W2009876395 @default.
• W2014148312 cites W2012840944 @default.
• W2014148312 cites W2023359102 @default.
• W2014148312 cites W2025431113 @default.
• W2014148312 cites W2032548513 @default.
• W2014148312 cites W2033825241 @default.
• W2014148312 cites W2035425048 @default.
• W2014148312 cites W2036758334 @default.
• W2014148312 cites W2042947978 @default.
• W2014148312 cites W2043038814 @default.
• W2014148312 cites W2063898064 @default.
• W2014148312 cites W2067167529 @default.
• W2014148312 cites W2072862994 @default.
• W2014148312 cites W2077193660 @default.
• W2014148312 cites W2078170734 @default.
• W2014148312 cites W2078906042 @default.
• W2014148312 cites W2083893624 @default.
• W2014148312 cites W2105329815 @default.
• W2014148312 cites W2106494572 @default.
• W2014148312 cites W2107267691 @default.
• W2014148312 cites W2112090747 @default.
• W2014148312 cites W2125809659 @default.
• W2014148312 cites W2128974582 @default.
• W2014148312 cites W2143466144 @default.
• W2014148312 cites W2146088499 @default.
• W2014148312 cites W2155606054 @default.
• W2014148312 cites W2157795161 @default.
• W2014148312 cites W2159839182 @default.
• W2014148312 cites W2169131251 @default.
• W2014148312 cites W3145335483 @default.
• W2014148312 doi "https://doi.org/10.1016/j.jmb.2012.11.008" @default.
• W2014148312 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5522729" @default.
• W2014148312 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23147217" @default.
• W2014148312 hasPublicationYear "2013" @default.
• W2014148312 type Work @default.
• W2014148312 sameAs 2014148312 @default.
• W2014148312 citedByCount "37" @default.
• W2014148312 countsByYear W20141483122013 @default.
• W2014148312 countsByYear W20141483122014 @default.
• W2014148312 countsByYear W20141483122015 @default.
• W2014148312 countsByYear W20141483122016 @default.
• W2014148312 countsByYear W20141483122017 @default.
• W2014148312 countsByYear W20141483122018 @default.
• W2014148312 countsByYear W20141483122019 @default.
• W2014148312 countsByYear W20141483122020 @default.
• W2014148312 countsByYear W20141483122022 @default.
• W2014148312 countsByYear W20141483122023 @default.
• W2014148312 crossrefType "journal-article" @default.
• W2014148312 hasAuthorship W2014148312A5012792532 @default.
• W2014148312 hasAuthorship W2014148312A5044773791 @default.
• W2014148312 hasAuthorship W2014148312A5063187030 @default.
• W2014148312 hasAuthorship W2014148312A5085424463 @default.
• W2014148312 hasBestOaLocation W20141483122 @default.
• W2014148312 hasConcept C104317684 @default.
• W2014148312 hasConcept C138885662 @default.
• W2014148312 hasConcept C179926584 @default.
• W2014148312 hasConcept C2776449523 @default.
• W2014148312 hasConcept C41895202 @default.
• W2014148312 hasConcept C54355233 @default.
• W2014148312 hasConcept C552990157 @default.
• W2014148312 hasConcept C67705224 @default.
• W2014148312 hasConcept C86803240 @default.
• W2014148312 hasConcept C95444343 @default.
• W2014148312 hasConceptScore W2014148312C104317684 @default.
• W2014148312 hasConceptScore W2014148312C138885662 @default.
• W2014148312 hasConceptScore W2014148312C179926584 @default.
• W2014148312 hasConceptScore W2014148312C2776449523 @default.
• W2014148312 hasConceptScore W2014148312C41895202 @default.
• W2014148312 hasConceptScore W2014148312C54355233 @default.
• W2014148312 hasConceptScore W2014148312C552990157 @default.
• W2014148312 hasConceptScore W2014148312C67705224 @default.
• W2014148312 hasConceptScore W2014148312C86803240 @default.
• W2014148312 hasConceptScore W2014148312C95444343 @default.
• W2014148312 hasIssue "1" @default.
• W2014148312 hasLocation W20141483121 @default.
• W2014148312 hasLocation W20141483122 @default.
• W2014148312 hasLocation W20141483123 @default.

• next