FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014150028> ?p ?o ?g. }

• W2014150028 endingPage "690" @default.
• W2014150028 startingPage "681" @default.
• W2014150028 abstract "Despite the advances in the understanding of biological processes, significant challenges still face those engaged in small molecule drug discovery. To complicate matters further, researchers are often overwhelmed with a range of off-the-shelf as well as bespoke assay formats to choose from when initiating a drug discovery programme. Although fluorescence intensity based assays have traditionally been adopted in drug discovery programmes for a wide range of target classes, it is essential to fully validate the chosen readouts to confirm that they accurately reflect the underlying biological mechanism under investigation.This review exemplifies the challenges that are often encountered with fluorescence intensity based assays and particular attention is paid to compound interference, the protease, deacetylating enzyme and kinase enzyme target classes.Designing a critical path in early stage drug discovery, which combines several diverse and minimally overlapping readout modes, will maximise the chance that compound activities will translate between the primary assay (utilised in the initial screening campaign) and secondary assay (utilised to evaluate the confirmed hits identified in the primary assay, usually a cell based assay) formats in a meaningful way. However, this is not always the case as is amply demonstrated across both academia and the pharmaceutical industry. Paying insufficient attention to these points can lead to the early termination of drug discovery programmes, not for want of resources or confidence in the rationale underlying the target, but instead because decision making has been driven by assay data originating from a different biological mechanism than the one under investigation.Although fluorescence intensity based assays are likely to remain popular for many target classes in drug discovery, in particular in small molecule screening campaigns, it is essential that at the outset they are sufficiently well validated so that compounds are likely to exhibit profiles that are confirmed in subsequent assays." @default.
• W2014150028 created "2016-06-24" @default.
• W2014150028 creator A5049765264 @default.
• W2014150028 creator A5052911277 @default.
• W2014150028 date "2010-06-15" @default.
• W2014150028 modified "2023-10-16" @default.
• W2014150028 title "Exemplification of the challenges associated with utilising fluorescence intensity based assays in discovery" @default.
• W2014150028 cites W141217221 @default.
• W2014150028 cites W1480141326 @default.
• W2014150028 cites W1588598829 @default.
• W2014150028 cites W1639582946 @default.
• W2014150028 cites W1968682237 @default.
• W2014150028 cites W1969334823 @default.
• W2014150028 cites W1977162423 @default.
• W2014150028 cites W1978880915 @default.
• W2014150028 cites W1981194888 @default.
• W2014150028 cites W1985469117 @default.
• W2014150028 cites W1985917720 @default.
• W2014150028 cites W1988805720 @default.
• W2014150028 cites W1991760227 @default.
• W2014150028 cites W1994848912 @default.
• W2014150028 cites W1997148748 @default.
• W2014150028 cites W1997319983 @default.
• W2014150028 cites W2006562777 @default.
• W2014150028 cites W2010082441 @default.
• W2014150028 cites W2012437459 @default.
• W2014150028 cites W2018951168 @default.
• W2014150028 cites W2027468355 @default.
• W2014150028 cites W2029149156 @default.
• W2014150028 cites W2034844930 @default.
• W2014150028 cites W2034993121 @default.
• W2014150028 cites W2036828264 @default.
• W2014150028 cites W2039957926 @default.
• W2014150028 cites W2041311068 @default.
• W2014150028 cites W2047187744 @default.
• W2014150028 cites W2054809740 @default.
• W2014150028 cites W2055571770 @default.
• W2014150028 cites W2056704728 @default.
• W2014150028 cites W2061843861 @default.
• W2014150028 cites W2062062228 @default.
• W2014150028 cites W2064352591 @default.
• W2014150028 cites W2066448033 @default.
• W2014150028 cites W2073113196 @default.
• W2014150028 cites W2081314213 @default.
• W2014150028 cites W2088381487 @default.
• W2014150028 cites W2089721697 @default.
• W2014150028 cites W2102643655 @default.
• W2014150028 cites W2103700658 @default.
• W2014150028 cites W2114733818 @default.
• W2014150028 cites W2116350182 @default.
• W2014150028 cites W2118527243 @default.
• W2014150028 cites W2118964113 @default.
• W2014150028 cites W2124072633 @default.
• W2014150028 cites W2128809047 @default.
• W2014150028 cites W2142802551 @default.
• W2014150028 cites W2157776316 @default.
• W2014150028 cites W2157860216 @default.
• W2014150028 cites W2159266419 @default.
• W2014150028 cites W2160860957 @default.
• W2014150028 cites W2164242115 @default.
• W2014150028 cites W2166550586 @default.
• W2014150028 cites W2792455800 @default.
• W2014150028 cites W2802869499 @default.
• W2014150028 cites W327746464 @default.
• W2014150028 cites W41086465 @default.
• W2014150028 cites W4231404455 @default.
• W2014150028 cites W4245731230 @default.
• W2014150028 doi "https://doi.org/10.1517/17460441.2010.495748" @default.
• W2014150028 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22823207" @default.
• W2014150028 hasPublicationYear "2010" @default.
• W2014150028 type Work @default.
• W2014150028 sameAs 2014150028 @default.
• W2014150028 citedByCount "21" @default.
• W2014150028 countsByYear W20141500282013 @default.
• W2014150028 countsByYear W20141500282014 @default.
• W2014150028 countsByYear W20141500282015 @default.
• W2014150028 countsByYear W20141500282017 @default.
• W2014150028 countsByYear W20141500282018 @default.
• W2014150028 countsByYear W20141500282019 @default.
• W2014150028 countsByYear W20141500282020 @default.
• W2014150028 countsByYear W20141500282021 @default.
• W2014150028 countsByYear W20141500282023 @default.
• W2014150028 crossrefType "journal-article" @default.
• W2014150028 hasAuthorship W2014150028A5049765264 @default.
• W2014150028 hasAuthorship W2014150028A5052911277 @default.
• W2014150028 hasConcept C112698675 @default.
• W2014150028 hasConcept C144133560 @default.
• W2014150028 hasConcept C2522767166 @default.
• W2014150028 hasConcept C41008148 @default.
• W2014150028 hasConcept C44210515 @default.
• W2014150028 hasConcept C60644358 @default.
• W2014150028 hasConcept C70721500 @default.
• W2014150028 hasConcept C74187038 @default.
• W2014150028 hasConcept C86803240 @default.
• W2014150028 hasConceptScore W2014150028C112698675 @default.
• W2014150028 hasConceptScore W2014150028C144133560 @default.
• W2014150028 hasConceptScore W2014150028C2522767166 @default.
• W2014150028 hasConceptScore W2014150028C41008148 @default.

• next