Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014157417> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2014157417 endingPage "196" @default.
- W2014157417 startingPage "185" @default.
- W2014157417 abstract "All metachromatically staining substances were originally thought by Lison (1935a; 1935b) to be the sulfuric esters of high-polymeric carbohydrates. Michaelis (1947) found that high-polymeric carbohydrates with carboxyl groups such as the capsular substances of pneumococci and mucin stain metachromatically (purple to red) with toluidine blue. Wiame (1946) found that metaphosphate also stains metachromatically. Both Wiame and Michaelis have shown by spectrophotometric analyses that metachromasy (when toluidine blue is used as the stain) is dependent upon (1) the concentration of metaphosphate, (2) the concentration of dye, and (3) the condition of the metaphosphate.The following tests devised by Grimme (1902) for the detection of volutin (metaphosphate), have been retained in general use: 1) metachromatic staining with dilute methylene blue, 2) extractability from unfixed cells with hot water at 80°C in five minutes, and 3) retention of the stain after treatment with 1 per cent sulfuric acid. Acid fastness is superior to the occurrence of metachromasy as a test for the presence of volutin.A cytological investigation of the location of metaphosphate (volutin) in the yeast cell was made in two types of preparations: 1) fixed impression smears and 2) progressively stained living cells. Essentially the same results were obtaned with both preparations except that the vacuoles were destroyed by drying the impression smears. The results showed that volutin (metaphosphate) is confined to the nuclear vacuole.There are three items which have led to erroneous conclusions concering the location of metaphosphate. The spindle, which is not visible in the unstained cell, often appears after staining as a clear area and is mistaken for the vacuole. Heavy deposits of glycogen similarly appear in the stained cell as clear areas and have been mistaken for the vacuole. The mitochondria, which are obvious in the cytoplasm, are destroyed by the staining and have been confused with the chromosomes covered with volutin which appear after staining." @default.
- W2014157417 created "2016-06-24" @default.
- W2014157417 creator A5008049409 @default.
- W2014157417 creator A5016127281 @default.
- W2014157417 creator A5047651778 @default.
- W2014157417 date "1955-01-01" @default.
- W2014157417 modified "2023-09-25" @default.
- W2014157417 title "The Location of Metaphosphate in the Yeast Cell" @default.
- W2014157417 cites W1551339674 @default.
- W2014157417 cites W1565108302 @default.
- W2014157417 cites W1970233240 @default.
- W2014157417 cites W1982779135 @default.
- W2014157417 cites W1984004741 @default.
- W2014157417 cites W1995292681 @default.
- W2014157417 cites W1998318241 @default.
- W2014157417 cites W2035537100 @default.
- W2014157417 cites W2037065638 @default.
- W2014157417 cites W2076608578 @default.
- W2014157417 cites W2082303664 @default.
- W2014157417 cites W2328932370 @default.
- W2014157417 cites W654080036 @default.
- W2014157417 doi "https://doi.org/10.1508/cytologia.20.185" @default.
- W2014157417 hasPublicationYear "1955" @default.
- W2014157417 type Work @default.
- W2014157417 sameAs 2014157417 @default.
- W2014157417 citedByCount "6" @default.
- W2014157417 crossrefType "journal-article" @default.
- W2014157417 hasAuthorship W2014157417A5008049409 @default.
- W2014157417 hasAuthorship W2014157417A5016127281 @default.
- W2014157417 hasAuthorship W2014157417A5047651778 @default.
- W2014157417 hasBestOaLocation W20141574171 @default.
- W2014157417 hasConcept C102568950 @default.
- W2014157417 hasConcept C152978389 @default.
- W2014157417 hasConcept C161790260 @default.
- W2014157417 hasConcept C178790620 @default.
- W2014157417 hasConcept C184710815 @default.
- W2014157417 hasConcept C185592680 @default.
- W2014157417 hasConcept C190062978 @default.
- W2014157417 hasConcept C2776335861 @default.
- W2014157417 hasConcept C2777132085 @default.
- W2014157417 hasConcept C2779191827 @default.
- W2014157417 hasConcept C2779262481 @default.
- W2014157417 hasConcept C2781294515 @default.
- W2014157417 hasConcept C54355233 @default.
- W2014157417 hasConcept C55493867 @default.
- W2014157417 hasConcept C65165184 @default.
- W2014157417 hasConcept C74864618 @default.
- W2014157417 hasConcept C86803240 @default.
- W2014157417 hasConceptScore W2014157417C102568950 @default.
- W2014157417 hasConceptScore W2014157417C152978389 @default.
- W2014157417 hasConceptScore W2014157417C161790260 @default.
- W2014157417 hasConceptScore W2014157417C178790620 @default.
- W2014157417 hasConceptScore W2014157417C184710815 @default.
- W2014157417 hasConceptScore W2014157417C185592680 @default.
- W2014157417 hasConceptScore W2014157417C190062978 @default.
- W2014157417 hasConceptScore W2014157417C2776335861 @default.
- W2014157417 hasConceptScore W2014157417C2777132085 @default.
- W2014157417 hasConceptScore W2014157417C2779191827 @default.
- W2014157417 hasConceptScore W2014157417C2779262481 @default.
- W2014157417 hasConceptScore W2014157417C2781294515 @default.
- W2014157417 hasConceptScore W2014157417C54355233 @default.
- W2014157417 hasConceptScore W2014157417C55493867 @default.
- W2014157417 hasConceptScore W2014157417C65165184 @default.
- W2014157417 hasConceptScore W2014157417C74864618 @default.
- W2014157417 hasConceptScore W2014157417C86803240 @default.
- W2014157417 hasIssue "2" @default.
- W2014157417 hasLocation W20141574171 @default.
- W2014157417 hasOpenAccess W2014157417 @default.
- W2014157417 hasPrimaryLocation W20141574171 @default.
- W2014157417 hasRelatedWork W1989005589 @default.
- W2014157417 hasRelatedWork W2014157417 @default.
- W2014157417 hasRelatedWork W2048591508 @default.
- W2014157417 hasRelatedWork W2057108164 @default.
- W2014157417 hasRelatedWork W2064156120 @default.
- W2014157417 hasRelatedWork W2137447885 @default.
- W2014157417 hasRelatedWork W2146526748 @default.
- W2014157417 hasRelatedWork W2409207753 @default.
- W2014157417 hasRelatedWork W2546656766 @default.
- W2014157417 hasRelatedWork W1617955388 @default.
- W2014157417 hasVolume "20" @default.
- W2014157417 isParatext "false" @default.
- W2014157417 isRetracted "false" @default.
- W2014157417 magId "2014157417" @default.
- W2014157417 workType "article" @default.