Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014161528> ?p ?o ?g. }
- W2014161528 endingPage "234" @default.
- W2014161528 startingPage "227" @default.
- W2014161528 abstract "'Candidatus Phytoplasma prunorum' is a wall-less bacterium associated with European stone fruit yellows (ESFY), a severe disease of Prunus spp. (mainly apricot and Japanese plum trees). It can be spread by one insect vector, Cacopsylla pruni, and by the trade of infected material. The availability of PCR-based methods allowing a sensitive and specific detection of 'Ca. P. prunorum' is crucial for this phytoplasma because, at present, it is uncultured and cannot be detected serologically. We developed a PCR test which, in contrast to the existing detection tools, provides a fast, specific and sensitive detection of 'Ca. P. prunorum' in plants and insects. For studies requiring an absolute quantification of the phytoplasma titer, the same primers were used to develop a real-time PCR assay, including a standard for C. pruni. The sensitivity of these molecular tools was compared by serial dilutions and their specificity was assessed both in silico and experimentally for reference strains and field samples of the closely related phytoplasma 'Ca. P. prunorum', 'Ca. P. pyri' (pear decline agent) and 'Ca. P. mali' (apple proliferation agent), as well as for representative strains of the 'Ca. Phytoplasma' genus." @default.
- W2014161528 created "2016-06-24" @default.
- W2014161528 creator A5043401891 @default.
- W2014161528 creator A5072849169 @default.
- W2014161528 creator A5080825877 @default.
- W2014161528 creator A5090719715 @default.
- W2014161528 date "2009-10-01" @default.
- W2014161528 modified "2023-10-02" @default.
- W2014161528 title "Specific detection and quantification of the phytopathogenic agent ‘Candidatus Phytoplasma prunorum’" @default.
- W2014161528 cites W1903206970 @default.
- W2014161528 cites W1976525668 @default.
- W2014161528 cites W1980198395 @default.
- W2014161528 cites W1981850574 @default.
- W2014161528 cites W1995026156 @default.
- W2014161528 cites W1996966171 @default.
- W2014161528 cites W1999528514 @default.
- W2014161528 cites W2010902539 @default.
- W2014161528 cites W2013364044 @default.
- W2014161528 cites W2018767931 @default.
- W2014161528 cites W2019428457 @default.
- W2014161528 cites W2022158322 @default.
- W2014161528 cites W2034786640 @default.
- W2014161528 cites W2035209674 @default.
- W2014161528 cites W2066002260 @default.
- W2014161528 cites W2071675782 @default.
- W2014161528 cites W2085068212 @default.
- W2014161528 cites W2091824434 @default.
- W2014161528 cites W2092386952 @default.
- W2014161528 cites W2099232951 @default.
- W2014161528 cites W2104936527 @default.
- W2014161528 cites W2106882534 @default.
- W2014161528 cites W2108535061 @default.
- W2014161528 cites W2121981718 @default.
- W2014161528 cites W2123843529 @default.
- W2014161528 cites W2143366038 @default.
- W2014161528 cites W2145409763 @default.
- W2014161528 cites W2145465491 @default.
- W2014161528 cites W2145816351 @default.
- W2014161528 cites W2149551170 @default.
- W2014161528 cites W2161121707 @default.
- W2014161528 cites W2290835555 @default.
- W2014161528 cites W252786129 @default.
- W2014161528 cites W2591357493 @default.
- W2014161528 cites W277426937 @default.
- W2014161528 doi "https://doi.org/10.1016/j.mcp.2009.04.005" @default.
- W2014161528 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19401232" @default.
- W2014161528 hasPublicationYear "2009" @default.
- W2014161528 type Work @default.
- W2014161528 sameAs 2014161528 @default.
- W2014161528 citedByCount "36" @default.
- W2014161528 countsByYear W20141615282012 @default.
- W2014161528 countsByYear W20141615282013 @default.
- W2014161528 countsByYear W20141615282014 @default.
- W2014161528 countsByYear W20141615282015 @default.
- W2014161528 countsByYear W20141615282016 @default.
- W2014161528 countsByYear W20141615282017 @default.
- W2014161528 countsByYear W20141615282018 @default.
- W2014161528 countsByYear W20141615282019 @default.
- W2014161528 countsByYear W20141615282020 @default.
- W2014161528 countsByYear W20141615282021 @default.
- W2014161528 countsByYear W20141615282022 @default.
- W2014161528 countsByYear W20141615282023 @default.
- W2014161528 crossrefType "journal-article" @default.
- W2014161528 hasAuthorship W2014161528A5043401891 @default.
- W2014161528 hasAuthorship W2014161528A5072849169 @default.
- W2014161528 hasAuthorship W2014161528A5080825877 @default.
- W2014161528 hasAuthorship W2014161528A5090719715 @default.
- W2014161528 hasConcept C104317684 @default.
- W2014161528 hasConcept C142724271 @default.
- W2014161528 hasConcept C168007829 @default.
- W2014161528 hasConcept C184112429 @default.
- W2014161528 hasConcept C204787440 @default.
- W2014161528 hasConcept C22508944 @default.
- W2014161528 hasConcept C2775874295 @default.
- W2014161528 hasConcept C2776988988 @default.
- W2014161528 hasConcept C2778533604 @default.
- W2014161528 hasConcept C49105822 @default.
- W2014161528 hasConcept C55493867 @default.
- W2014161528 hasConcept C59822182 @default.
- W2014161528 hasConcept C71924100 @default.
- W2014161528 hasConcept C86803240 @default.
- W2014161528 hasConcept C89423630 @default.
- W2014161528 hasConceptScore W2014161528C104317684 @default.
- W2014161528 hasConceptScore W2014161528C142724271 @default.
- W2014161528 hasConceptScore W2014161528C168007829 @default.
- W2014161528 hasConceptScore W2014161528C184112429 @default.
- W2014161528 hasConceptScore W2014161528C204787440 @default.
- W2014161528 hasConceptScore W2014161528C22508944 @default.
- W2014161528 hasConceptScore W2014161528C2775874295 @default.
- W2014161528 hasConceptScore W2014161528C2776988988 @default.
- W2014161528 hasConceptScore W2014161528C2778533604 @default.
- W2014161528 hasConceptScore W2014161528C49105822 @default.
- W2014161528 hasConceptScore W2014161528C55493867 @default.
- W2014161528 hasConceptScore W2014161528C59822182 @default.
- W2014161528 hasConceptScore W2014161528C71924100 @default.
- W2014161528 hasConceptScore W2014161528C86803240 @default.
- W2014161528 hasConceptScore W2014161528C89423630 @default.
- W2014161528 hasIssue "5" @default.