Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014176021> ?p ?o ?g. }
- W2014176021 endingPage "4843" @default.
- W2014176021 startingPage "4836" @default.
- W2014176021 abstract "A growing list of proteins, including the β-sheet-rich SH3 domain, is known to transiently populate a compact α-helical intermediate before settling into the native structure. Examples have been discovered in cryogenic solvent as well as by pressure jumps. Earlier studies of λ repressor mutants showed that transient states with excess helix are robust in an all-α protein. Here we extend a previous study of src SH3 domain to two new SH3 sequences, phosphatidylinositol 3-kinase (PI3K) and a Fyn mutant, to see how robust such helix-rich transients are to sequence variations in this β-sheet fold. We quantify helical structure by circular dichroism (CD), protein compactness by small-angle X-ray scattering (SAXS), and transient helical populations by cryo-stopped-flow CD. Our results show that transient compact helix-rich intermediates are easily accessible on the folding landscape of different SH3 domains. In molecular dynamics simulations, force field errors are often blamed for transient non-native structure. We suggest that experimental examples of very fast α-rich transient misfolding could become a more subtle test for further force field improvements than observation of the native state alone." @default.
- W2014176021 created "2016-06-24" @default.
- W2014176021 creator A5004911196 @default.
- W2014176021 creator A5009810193 @default.
- W2014176021 creator A5013859727 @default.
- W2014176021 creator A5026054845 @default.
- W2014176021 creator A5075822016 @default.
- W2014176021 creator A5091107302 @default.
- W2014176021 date "2013-04-18" @default.
- W2014176021 modified "2023-09-23" @default.
- W2014176021 title "Transient Helical Structure during PI3K and Fyn SH3 Domain Folding" @default.
- W2014176021 cites W1510728289 @default.
- W2014176021 cites W1593625064 @default.
- W2014176021 cites W1975410659 @default.
- W2014176021 cites W1981192327 @default.
- W2014176021 cites W1984903816 @default.
- W2014176021 cites W1986863252 @default.
- W2014176021 cites W1990584220 @default.
- W2014176021 cites W1990593157 @default.
- W2014176021 cites W1994239510 @default.
- W2014176021 cites W1999249276 @default.
- W2014176021 cites W2006520052 @default.
- W2014176021 cites W2007886752 @default.
- W2014176021 cites W2010512829 @default.
- W2014176021 cites W2017394797 @default.
- W2014176021 cites W2018615568 @default.
- W2014176021 cites W2020353121 @default.
- W2014176021 cites W2026101733 @default.
- W2014176021 cites W2028660129 @default.
- W2014176021 cites W2028672605 @default.
- W2014176021 cites W2032418563 @default.
- W2014176021 cites W2037159954 @default.
- W2014176021 cites W2041811349 @default.
- W2014176021 cites W2045731402 @default.
- W2014176021 cites W2052727298 @default.
- W2014176021 cites W2053058256 @default.
- W2014176021 cites W2053315180 @default.
- W2014176021 cites W2054111071 @default.
- W2014176021 cites W2054643353 @default.
- W2014176021 cites W2056559191 @default.
- W2014176021 cites W2058359968 @default.
- W2014176021 cites W2060915934 @default.
- W2014176021 cites W2072157747 @default.
- W2014176021 cites W2072516324 @default.
- W2014176021 cites W2072927989 @default.
- W2014176021 cites W2073656813 @default.
- W2014176021 cites W2075861371 @default.
- W2014176021 cites W2078177368 @default.
- W2014176021 cites W2078838993 @default.
- W2014176021 cites W2081486996 @default.
- W2014176021 cites W2082719343 @default.
- W2014176021 cites W2082849614 @default.
- W2014176021 cites W2092779242 @default.
- W2014176021 cites W2093540297 @default.
- W2014176021 cites W2105717859 @default.
- W2014176021 cites W2107876005 @default.
- W2014176021 cites W2115953328 @default.
- W2014176021 cites W2116076986 @default.
- W2014176021 cites W4293226720 @default.
- W2014176021 doi "https://doi.org/10.1021/jp400167s" @default.
- W2014176021 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3841067" @default.
- W2014176021 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23537292" @default.
- W2014176021 hasPublicationYear "2013" @default.
- W2014176021 type Work @default.
- W2014176021 sameAs 2014176021 @default.
- W2014176021 citedByCount "13" @default.
- W2014176021 countsByYear W20141760212014 @default.
- W2014176021 countsByYear W20141760212015 @default.
- W2014176021 countsByYear W20141760212016 @default.
- W2014176021 countsByYear W20141760212017 @default.
- W2014176021 countsByYear W20141760212018 @default.
- W2014176021 countsByYear W20141760212019 @default.
- W2014176021 countsByYear W20141760212020 @default.
- W2014176021 countsByYear W20141760212021 @default.
- W2014176021 crossrefType "journal-article" @default.
- W2014176021 hasAuthorship W2014176021A5004911196 @default.
- W2014176021 hasAuthorship W2014176021A5009810193 @default.
- W2014176021 hasAuthorship W2014176021A5013859727 @default.
- W2014176021 hasAuthorship W2014176021A5026054845 @default.
- W2014176021 hasAuthorship W2014176021A5075822016 @default.
- W2014176021 hasAuthorship W2014176021A5091107302 @default.
- W2014176021 hasBestOaLocation W20141760212 @default.
- W2014176021 hasConcept C108636557 @default.
- W2014176021 hasConcept C133571119 @default.
- W2014176021 hasConcept C147597530 @default.
- W2014176021 hasConcept C184235292 @default.
- W2014176021 hasConcept C185592680 @default.
- W2014176021 hasConcept C18903297 @default.
- W2014176021 hasConcept C196347352 @default.
- W2014176021 hasConcept C203087120 @default.
- W2014176021 hasConcept C204328495 @default.
- W2014176021 hasConcept C2778530040 @default.
- W2014176021 hasConcept C2779965526 @default.
- W2014176021 hasConcept C47701112 @default.
- W2014176021 hasConcept C55493867 @default.
- W2014176021 hasConcept C59593255 @default.
- W2014176021 hasConcept C8010536 @default.
- W2014176021 hasConcept C86803240 @default.