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- W2014204043 abstract "A simple procedure is described for removing the vitelline membrane of sea urchin eggs. The method is based on the observation that brief incubation of unfertilized sea urchin eggs in 5–10 mM dithiothreitol (DTT or Cleland Reagent) at pH 9.2 effectively removes this membrane. Membrane removal and normal development have been obtained with the eggs of the sea urchins Strongylocentrotus purpuratus and Lytechinus pictus. That DTT removes membranes, combined with other observations and experiments, indicates that the structural integrity of the vitelline membrane results in part from disulfide linkages between neighboring protein molecules." @default.
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- W2014204043 date "1950-01-01" @default.
- W2014204043 modified "2023-09-27" @default.
- W2014204043 title "Action of ficin on sea urchin eggs" @default.
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- W2014204043 doi "https://doi.org/10.1016/0014-4827(50)90044-9" @default.
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