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• W2014212917 abstract "Four different H-type 1 (LedH) blood-group-active glycosphingolipids (LedH-I–IV) have been isolated from the plasma of blood-group O Le(a−b−) secretors. The agglutination of O Le(a−b−) erythrocytes from secretors by 50 μl of 4 hemagglutinating units of caprine anti-LedH (anti-H-type 1) serum was inhibited by 0.02 μg of each of all four glycolipids. No Lea or Leb activities or reaction against Ulex europaeus lectin could be found. LedH-I, -II, -III, and -IV at 0.05, 0.01, 0.01, and 0.02 μg each are sufficient for incubation in order to convert 9 × 107 O Le(a−b−) erythrocytes from nonsecretors into H-type 1 (LedH)-positive cells. Structural analysis of the H-type 1 glycolipids was performed in comparison to that of Lea- and Leb-blood-group-active glycolipids from human plasma isolated previously: Gas chromatography of peracetylated alditols revealed sugar composition. Combined gas chromatography-mass spectrometry established the glycosidic linkages. Together with the results obtained by direct inlet mass spectrometry of permethylated glycosphingolipids and by 360-MHz 1H nuclear magnetic resonance spectroscopy (Egge, H., and Hanfland, P., 1981, Arch. Biochem. Biophys., 210, 396–404; Dabrowski, J., Hanfland, P., Egge, H., and Dabrowski, U., 1981, Arch. Biochem. Biophys., 210, 405–411) the complete structures of the oligosaccharide chains of the Lea-, Leb-, and H-type 1-active glycolipids were established: Galβ1 → 3GlcNAc(4 ← 1αFuc)β1 → 3Galβ1 → 4Glcβ1 → 1 Cer for the Lea antigens; Fucα1 → 2Galβ1 → 3GlcNAc(4 ← 1αFuc)β1 → 3Galβ1 → 4Glcβ1 → 1 Cer for the Leb antigens; and Fucα1 → 2Galβ1 → 3GlcNAcβ1 → 3Galβ1 → 4Glcβ1 → 1 Cer for the H-type 1 (LedH) glycolipids. The diverse antigens of the same blood-group specificity obviously differ from one another in their lipid residue. In addition, plasmatic neolactotetraosylceramide could be identified, differing from that of human erythrocytes by a slower migration behavior in thin-layer chromatography." @default.
• W2014212917 created "2016-06-24" @default.
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• W2014212917 date "1981-08-01" @default.
• W2014212917 modified "2023-10-17" @default.
• W2014212917 title "Immunochemistry of the Lewis-blood-group system: Partial characterization of Lea-, Leb-, and H-type 1 (LedH)-blood-group active glycosphingolipids from human plasma" @default.
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• W2014212917 doi "https://doi.org/10.1016/0003-9861(81)90201-0" @default.
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