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- W2014230041 abstract "Abstract Protoplasts were enzymatically released from somatic embryos of carrot and were cultured in a liquid medium and an agar plate. Addition of 10−7 M 2,4-dichlorophenoxyacetic acid (2,4-D) to the modified Lin and Staba medium kept most of the isolated protoplasts more healthy. About 10% of the isolated protoplasts divided once within a week, but they did not proliferate continuously. In a medium without 2,4-D, on the other hand, about 0.1% of the embryo-protoplasts began to divide continuously after 2–3 weeks. They again formed cell clusters and somatic embryos, but slowly. Cell contact or aggregation was found to be effective for isolated embryo- protoplasts to maintain their original appearance and to proliferate continuously. Without contact with other cells, most of the cultured protoplasts formed highly vacuolated, very large cells without proliferation." @default.
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- W2014230041 date "1983-03-01" @default.
- W2014230041 modified "2023-09-25" @default.
- W2014230041 title "Culture of protoplasts isolated from somatic embryos of carrot" @default.
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- W2014230041 doi "https://doi.org/10.1016/0304-4211(83)90017-2" @default.
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