Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014232471> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2014232471 endingPage "189" @default.
- W2014232471 startingPage "178" @default.
- W2014232471 abstract "Abstract Commercial tomato pectin methylesterase has been separated into 4–6 protein fractions by gel filtration on Sephadex G-75 and G-200. The molecular weights of these fractions ranged from 6800 to more than 235 000. The enzyme was localized in only one fraction, in which the distribution of enzyme activity essentially paralleled that of ultraviolet absorbance. On analytical column gel filtration and on thin-layer gel filtration on Sephadex G-75, the molecular weight of the enzyme was found to be 24 000 and 27 000, respectively. A possible interaction of the enzyme with the dextran gel was excluded by thin-layer gel filtration on the polyacrylamide Bio-Gel P-60, a molecular weight of 28 000 being obtained. Ultracentrifugal analysis of the enzyme isolated by preparative gel filtration followed by (NH 4 ) 2 SO 4 precipitation gave an s 0 20, w value of 3.3 S. The molecular weight determined by sedimentation equilibrium was 26 300. The frictional ratio was calculated to be 1.17. Electrophoretic heterogeneity of the commercial enzyme preparation and of some of the fractions isolated by preparative gel filtration was demonstrated by paper electrophoresis. The isoelectric point of pectin methylesterase was found to be 8.4 at 20° by thin-layer isoelectric focusing." @default.
- W2014232471 created "2016-06-24" @default.
- W2014232471 creator A5036958128 @default.
- W2014232471 creator A5058189766 @default.
- W2014232471 date "1970-07-01" @default.
- W2014232471 modified "2023-09-29" @default.
- W2014232471 title "Some size and charge properties of tomato pectin methylesterase" @default.
- W2014232471 cites W1968083082 @default.
- W2014232471 cites W1984227451 @default.
- W2014232471 cites W2012102268 @default.
- W2014232471 cites W2016478786 @default.
- W2014232471 cites W2043762391 @default.
- W2014232471 cites W2048875921 @default.
- W2014232471 cites W2083945806 @default.
- W2014232471 cites W2090257040 @default.
- W2014232471 cites W2095046438 @default.
- W2014232471 cites W2137302959 @default.
- W2014232471 cites W2168799653 @default.
- W2014232471 doi "https://doi.org/10.1016/0005-2795(70)90082-6" @default.
- W2014232471 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5496095" @default.
- W2014232471 hasPublicationYear "1970" @default.
- W2014232471 type Work @default.
- W2014232471 sameAs 2014232471 @default.
- W2014232471 citedByCount "36" @default.
- W2014232471 crossrefType "journal-article" @default.
- W2014232471 hasAuthorship W2014232471A5036958128 @default.
- W2014232471 hasAuthorship W2014232471A5058189766 @default.
- W2014232471 hasConcept C109214941 @default.
- W2014232471 hasConcept C121332964 @default.
- W2014232471 hasConcept C127413603 @default.
- W2014232471 hasConcept C172854840 @default.
- W2014232471 hasConcept C181199279 @default.
- W2014232471 hasConcept C185592680 @default.
- W2014232471 hasConcept C188082385 @default.
- W2014232471 hasConcept C192562407 @default.
- W2014232471 hasConcept C2778189321 @default.
- W2014232471 hasConcept C2780756937 @default.
- W2014232471 hasConcept C31903555 @default.
- W2014232471 hasConcept C42360764 @default.
- W2014232471 hasConcept C55493867 @default.
- W2014232471 hasConceptScore W2014232471C109214941 @default.
- W2014232471 hasConceptScore W2014232471C121332964 @default.
- W2014232471 hasConceptScore W2014232471C127413603 @default.
- W2014232471 hasConceptScore W2014232471C172854840 @default.
- W2014232471 hasConceptScore W2014232471C181199279 @default.
- W2014232471 hasConceptScore W2014232471C185592680 @default.
- W2014232471 hasConceptScore W2014232471C188082385 @default.
- W2014232471 hasConceptScore W2014232471C192562407 @default.
- W2014232471 hasConceptScore W2014232471C2778189321 @default.
- W2014232471 hasConceptScore W2014232471C2780756937 @default.
- W2014232471 hasConceptScore W2014232471C31903555 @default.
- W2014232471 hasConceptScore W2014232471C42360764 @default.
- W2014232471 hasConceptScore W2014232471C55493867 @default.
- W2014232471 hasIssue "1" @default.
- W2014232471 hasLocation W20142324711 @default.
- W2014232471 hasLocation W20142324712 @default.
- W2014232471 hasOpenAccess W2014232471 @default.
- W2014232471 hasPrimaryLocation W20142324711 @default.
- W2014232471 hasRelatedWork W1974442974 @default.
- W2014232471 hasRelatedWork W1989730545 @default.
- W2014232471 hasRelatedWork W2004940341 @default.
- W2014232471 hasRelatedWork W2020244894 @default.
- W2014232471 hasRelatedWork W2020925881 @default.
- W2014232471 hasRelatedWork W2049333627 @default.
- W2014232471 hasRelatedWork W2094864026 @default.
- W2014232471 hasRelatedWork W2148068466 @default.
- W2014232471 hasRelatedWork W276312987 @default.
- W2014232471 hasRelatedWork W2579576610 @default.
- W2014232471 hasVolume "214" @default.
- W2014232471 isParatext "false" @default.
- W2014232471 isRetracted "false" @default.
- W2014232471 magId "2014232471" @default.
- W2014232471 workType "article" @default.