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• W2014232711 abstract "To examine the expression patterns of the triggering receptor expressed on myeloid cells (TREM)-1 during experimental septic shock.Animal study.Animal research laboratory.Male BALB/c mice, 7-9 wks of age.Septic shock was induced by cecal ligation and puncture in eight mice. Eight additional animals were sham-operated and served as a control group. All animals were resuscitated by fluid infusion and administered antibiotics. Kill was performed under anesthesia 12, 24, or 48 hrs later.Surface expression of TREM-1 was analyzed using flow cytometry on peripheral blood cells, peritoneal macrophages and neutrophils, splenic macrophages, and Kupffer cells. Gene expression was also studied in these same cells using reverse transcription-polymerase chain reaction. Tumor necrosis factor-alpha, interleukin-1beta, and soluble TREM-1 concentrations were determined in plasma and peritoneal lavage fluid. Sepsis strongly induced TREM-1 gene expression, which translated into an up-regulation of TREM-1 surface expression on neutrophils and monocytes/macrophages both at the focus on infection as well as distally. Moreover, sepsis induced the release of significant levels of soluble TREM-1. Plasma soluble TREM-1 concentrations negatively correlated with tumor necrosis factor-alpha and interleukin-1beta levels at 12 hrs.These results provide new information as to the regulation of TREM-1 during sepsis. Considering that both cell-surface and soluble TREM-1 were strongly up-regulated during infection, this study may add support to the putative usefulness of TREM-1 as a diagnostic tool." @default.
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• W2014232711 date "2005-08-01" @default.
• W2014232711 modified "2023-10-13" @default.
• W2014232711 title "Surface and soluble triggering receptor expressed on myeloid cells-1: Expression patterns in murine sepsis" @default.
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• W2014232711 doi "https://doi.org/10.1097/01.ccm.0000172614.36571.75" @default.
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