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- W2014237802 abstract "A reversed phase high performance liquid chromatographic method for the determination of danshensu, protocatechuic acid, protocatechualdehyde, (+)-catechin, and paeoniflorin in traditional Chinese medicinal preparation Shandanshaoyao Tang was established. Danshensu, protocatechuic acid, protocatechualdehyde, and (+)-catechin were separated on a Diamonsil C18 column (250 mm × 4.6 mm I.D.) with a mobile phase consisting of methanol-acetic acid-water (14: 0.2: 86, v/v), and detected by UV at 280 nm. Paeoniflorin was separated using the same column but with a mobile phase consisting of methanol-acetic acid-water (25: 0.2: 75, v/v), and detected by UV at 230 nm. The linear calibration ranges were 24.1–241.0 μg/mL, 6.1–61.0 μg/mL, 2.5–25.4 μg/mL, 8.3–83.0 μg/mL and 224–2235 μg/mL for danshensu, protocatechuic acid, protocatechualdehyde, (+)-catechin, and paeoniflorin, respectively. The relative standard deviations (n=5) and average recoveries (n=5) were 1.45% and 91.4% for danshensu, 1.54% and 100.1% for protocatechuic acid, 0.89% and 95.3% for protocatechualdehyde, 1.78% and 93.6% for (+)-catechin, and 0.68% and 95.3% for paeoniflorin, respectively. The contents of these five bioactive constituents in Shandanshaoyao Tang were successfully determined by the proposed method." @default.
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- W2014237802 date "2001-08-31" @default.
- W2014237802 modified "2023-09-26" @default.
- W2014237802 title "DETERMINATION OF FIVE BIOACTIVE CONSTITUENTS IN TRADITIONAL CHINESE MEDICINAL PREPARATION SHANDANSHAOYAO TANG BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY" @default.
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- W2014237802 doi "https://doi.org/10.1081/jlc-100104444" @default.
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