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- W2014248309 abstract "The aim of this work was to characterize the individual contribution of the amnion (AMN) and choriodecidua (CHD) regions to the secretion of human beta defensins (HBD)-1, -2, and -3, after stimulation with Streptococcus agalactiae.Full-thickness membranes were mounted on a Transwell device, constituted by two independent chambers; 1 × 10(6) CFU/ml of S. agalactiae were added to either the AMN or CHD face or to both. Secretion profiles of HBD-1, HBD-2, and HBD-3 to the culture medium were quantified by enzyme-linked immunosorbent sandwich assay (ELISA).Secretion profile of HBD-1 remained without significant changes; HBD-2 secretion level by the CHD increased 2.0 (2.73 ± 0.19 pg/μg) and 2.6 (3.62 ± 0.60 pg/μg) times when the stimulus was applied only to the CHD region and simultaneously to both compartments, respectively. The bacterial stimulation in the AMN induced a 2.0 times (2.06 ± 0.29 pg/μg) increase in this region. HBD-3 secretion level increased significantly in the CHD (15.65 ± 2.68 pg/μg) and the AMN (14.94 ± 1.85 pg/μg) only when both regions were stimulated simultaneously.The stimulation of human fetal membranes with S. agalactiae induced a differential and tissue-specific profile of HBD-1, HBD-2, and HBD-3 secretion." @default.
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- W2014248309 date "2011-06-01" @default.
- W2014248309 modified "2023-10-17" @default.
- W2014248309 title "Evidence of in vitro differential secretion of human beta-defensins-1, -2, and -3 after selective exposure to Streptococcus agalactiae in human fetal membranes" @default.
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- W2014248309 doi "https://doi.org/10.3109/14767058.2011.578695" @default.
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