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- W2014253934 abstract "Experiments were performed to determine whether androgens, in enhancing estrogen secretion in rat granulosacells, act solely as substrates for FSH-induced aromataseactivity or whether they also participate in the mechanism bywhich FSH increases the activity of the enzyme. Granulosa cellswere isolated from 27-day-old rats and cultured during a 24- or36-h induction period in the presence of FSH (0.5 μg/ml MRSFSHor 1.0 μg/ml NIH-FSH-Sll) with or without testosterone(0.5 μM) or other steroids. The cells were then cultured for afurther 6- to 12-h test period in medium containing no addedhormones or testosterone, an aromatizable androgen. The production of 17β-estradiol during the test period was measured as an index of aromatase activity stimulated by treatments during the induction period. In the absence of FSH during the induction period, estrogen biosynthesis was very low during both induction and test periods. The addition of FSH alone during the induction period resulted in significantly increased estroge..." @default.
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- W2014253934 title "Enhancement of Follicle-Stimulating Hormone-Induced Aromatase Activity by Androgens in Cultured Rat Granulosa Cells*" @default.
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- W2014253934 doi "https://doi.org/10.1210/endo-107-4-1027" @default.
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