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• W2014265002 abstract "SummaryProflavine bound-superhelical ϕXRFI DNA molecules undergo single-strand scissions upon irradiation with visible light at high fluence rate. As shown by agarose gel electrophoresis analyses, the nicking reaction is (i) oxygen-dependent, (ii) strongly inhibited by catalase and an electron scavenger such as cystamine, and (iii) totally suppressed by ceruloplasmin and radical scavengers such as t-butanol and sodium benzoate. This indicates that H2O2, e−, O and OH·, respectively, are involved in the cleavage process. NaN3, a singlet-oxygen quencher, has very little effect on strand-breakage but it prevents almost completely the alteration of guanine residues (a lesion already observed after irradiation at low fluence rate). Since, in the presence of NaN3, strand scission can occur and guanine (as the other bases) recovered intact, it follows that the radical intermediates produced during breakages are probably not involved in any permanent modification of the DNA bases." @default.
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• W2014265002 date "1981-01-01" @default.
• W2014265002 modified "2023-09-30" @default.
• W2014265002 title "Mechanism for Strand-break Induction in DNA–proflavine Complexes Exposed to Visible Light" @default.
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• W2014265002 doi "https://doi.org/10.1080/09553008114551371" @default.
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