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- W2014272254 abstract "Porphyromonas gingivalis expresses several virulence factors such as fimbriae and proteases, termed gingipains, which are enzymes that process precursor fimbriae proteins. Thus, gingipain-null mutants lack mature fimbriae. Membrane vesicle-depleted supernatants (VDS) containing soluble gingipains were prepared as an exogenous gingipain fraction. Precursor proteins were treated with VDS and a fimbriated gingipain-null mutant was successfully generated. Experiments showed that the wild strain adhered to and invaded epithelial cells at a greater level than the fimbriated gingipain-null mutant, while adhesion/invasion was prevented in the presence of fetal calf serum, which inhibits gingipain activity. The findings of this study suggest that gingipains expose cellular cryptic ligands in a proteolytic manner and promote fimbriae binding to epithelial cells." @default.
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- W2014272254 date "2007-08-01" @default.
- W2014272254 modified "2023-10-11" @default.
- W2014272254 title "Maturation of fimbria precursor protein by exogenous gingipains in<i>Porphyromonas gingivalis</i>gingipain-null mutant" @default.
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- W2014272254 doi "https://doi.org/10.1111/j.1574-6968.2007.00779.x" @default.
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