Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014277218> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2014277218 abstract "Early events in the cellular synthesis and subsequent transfer into membrane-limited compartments of pre-proparathyroid hormone (pre-proPTH) and proparathyroid hormone (proPTH) were investigated by electrophoretic analyses of newly synthesized proteins in subcellular fractions of parthyroid gland slices pulse-labeled for 0.5-5 min with [(35)S] methionine. During these short times of incubation, both pre-proPTH and proPTH were confined to the microsomal fraction. Labeled pre-proPTH and proPTH were detected in a 30-s interval between 0.5 and 1.0 min of incubation. The radioactivity in proPTH became relatively constant between 3 and 5 min, whereas the radioactivity in ProPTH increased markedly over this period. When corrected for the known content of methionine in the prohormone and the prohormone, we found four times as much radiolabeled prohormone as prehormone between 0.5 and 1.0 min of synthesis. Sequestration of labeled prohomrone into endoplasmic reticulum compartments was shown by treatment of the microsomal fraction with chymotrypsin and trypsin, which resulted in the degradation of the prehormone but not of the prohormones. Approximately 50 percent of pre-prohormone and 25 percent of prohormone were released from the microsomes by their extraction with 1.0 M KCl, whereas 80-90 percent of both was released by treatment with Triton X-100. These results in intact cells support the signal hypothesis proposed by Blobel and his co-workers in studies utilizing cell-free systems, inasmuch as the results indicate transfer of prohormone into the cisternal space of the rough endoplasmic reticulum concomitant with the growth of the nascent polypeptide chain. Appearance of membrane-sequestered proPTH takes place without entry of pre-proPTH into the cisternal space, suggesting that proteolytic removal of the leader peptide occurs during transfer of the polypeptide through the lipid bilayer. Further evidence in support of this process is that pre-proPTH is only partly extracted from the microsomes by treatment with 1.0 M KCl, suggesting that a substantial fraction of the nascent pre-proPTH is integrally inserted into the membranes before it is cleaved to form proPTH." @default.
- W2014277218 created "2016-06-24" @default.
- W2014277218 creator A5024986014 @default.
- W2014277218 creator A5039482644 @default.
- W2014277218 creator A5042773219 @default.
- W2014277218 creator A5086865530 @default.
- W2014277218 date "1980-05-01" @default.
- W2014277218 modified "2023-10-15" @default.
- W2014277218 title "Early events in the cellular formation of proparathyroid hormone" @default.
- W2014277218 cites W1517005261 @default.
- W2014277218 cites W1652524805 @default.
- W2014277218 cites W2015295302 @default.
- W2014277218 cites W2017032159 @default.
- W2014277218 cites W2018289835 @default.
- W2014277218 cites W2036538686 @default.
- W2014277218 cites W2041205318 @default.
- W2014277218 cites W2046595569 @default.
- W2014277218 cites W2050702527 @default.
- W2014277218 cites W2061790325 @default.
- W2014277218 cites W2064354624 @default.
- W2014277218 cites W2077022733 @default.
- W2014277218 cites W2100837269 @default.
- W2014277218 cites W2134817259 @default.
- W2014277218 cites W2145045711 @default.
- W2014277218 cites W2146923243 @default.
- W2014277218 cites W2278402341 @default.
- W2014277218 cites W2417720451 @default.
- W2014277218 doi "https://doi.org/10.1083/jcb.85.2.292" @default.
- W2014277218 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2110632" @default.
- W2014277218 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7372710" @default.
- W2014277218 hasPublicationYear "1980" @default.
- W2014277218 type Work @default.
- W2014277218 sameAs 2014277218 @default.
- W2014277218 citedByCount "26" @default.
- W2014277218 countsByYear W20142772182012 @default.
- W2014277218 countsByYear W20142772182016 @default.
- W2014277218 countsByYear W20142772182017 @default.
- W2014277218 countsByYear W20142772182018 @default.
- W2014277218 countsByYear W20142772182021 @default.
- W2014277218 countsByYear W20142772182022 @default.
- W2014277218 countsByYear W20142772182023 @default.
- W2014277218 crossrefType "journal-article" @default.
- W2014277218 hasAuthorship W2014277218A5024986014 @default.
- W2014277218 hasAuthorship W2014277218A5039482644 @default.
- W2014277218 hasAuthorship W2014277218A5042773219 @default.
- W2014277218 hasAuthorship W2014277218A5086865530 @default.
- W2014277218 hasBestOaLocation W20142772181 @default.
- W2014277218 hasConcept C119062480 @default.
- W2014277218 hasConcept C126322002 @default.
- W2014277218 hasConcept C153911025 @default.
- W2014277218 hasConcept C158617107 @default.
- W2014277218 hasConcept C181199279 @default.
- W2014277218 hasConcept C202751555 @default.
- W2014277218 hasConcept C25642318 @default.
- W2014277218 hasConcept C2780090379 @default.
- W2014277218 hasConcept C2780340462 @default.
- W2014277218 hasConcept C2780912031 @default.
- W2014277218 hasConcept C515207424 @default.
- W2014277218 hasConcept C55493867 @default.
- W2014277218 hasConcept C71315377 @default.
- W2014277218 hasConcept C71924100 @default.
- W2014277218 hasConcept C86803240 @default.
- W2014277218 hasConcept C87644729 @default.
- W2014277218 hasConceptScore W2014277218C119062480 @default.
- W2014277218 hasConceptScore W2014277218C126322002 @default.
- W2014277218 hasConceptScore W2014277218C153911025 @default.
- W2014277218 hasConceptScore W2014277218C158617107 @default.
- W2014277218 hasConceptScore W2014277218C181199279 @default.
- W2014277218 hasConceptScore W2014277218C202751555 @default.
- W2014277218 hasConceptScore W2014277218C25642318 @default.
- W2014277218 hasConceptScore W2014277218C2780090379 @default.
- W2014277218 hasConceptScore W2014277218C2780340462 @default.
- W2014277218 hasConceptScore W2014277218C2780912031 @default.
- W2014277218 hasConceptScore W2014277218C515207424 @default.
- W2014277218 hasConceptScore W2014277218C55493867 @default.
- W2014277218 hasConceptScore W2014277218C71315377 @default.
- W2014277218 hasConceptScore W2014277218C71924100 @default.
- W2014277218 hasConceptScore W2014277218C86803240 @default.
- W2014277218 hasConceptScore W2014277218C87644729 @default.
- W2014277218 hasLocation W20142772181 @default.
- W2014277218 hasLocation W20142772182 @default.
- W2014277218 hasLocation W20142772183 @default.
- W2014277218 hasLocation W20142772184 @default.
- W2014277218 hasOpenAccess W2014277218 @default.
- W2014277218 hasPrimaryLocation W20142772181 @default.
- W2014277218 hasRelatedWork W134525808 @default.
- W2014277218 hasRelatedWork W1539789931 @default.
- W2014277218 hasRelatedWork W1991980875 @default.
- W2014277218 hasRelatedWork W2007854243 @default.
- W2014277218 hasRelatedWork W2014277218 @default.
- W2014277218 hasRelatedWork W2014614188 @default.
- W2014277218 hasRelatedWork W2021572552 @default.
- W2014277218 hasRelatedWork W2081414589 @default.
- W2014277218 hasRelatedWork W2097373308 @default.
- W2014277218 hasRelatedWork W2317501046 @default.
- W2014277218 isParatext "false" @default.
- W2014277218 isRetracted "false" @default.
- W2014277218 magId "2014277218" @default.
- W2014277218 workType "article" @default.