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• W2014277911 abstract "Homing endonucleases, endonucleases capable of recognizing long DNA sequences, have been shown to be a tool of choice for precise and efficient genome engineering. Consequently, the possibility to engineer novel endonucleases with tailored specificities is under strong investigation. In this report, we present a simple and efficient method to select meganucleases from libraries of variants, based on their cleavage properties. The method has the advantage of directly selecting for the ability to induce double-strand break induced homologous recombination in a eukaryotic environment. Model selections demonstrated high levels of enrichments. Moreover, this method compared favorably with phage display for enrichment of active mutants from a mutant library. This approach makes possible the exploration of large sequence spaces and thereby represents a valuable tool for genome engineering." @default.
• W2014277911 created "2016-06-24" @default.
• W2014277911 creator A5053223873 @default.
• W2014277911 date "2005-11-27" @default.
• W2014277911 modified "2023-09-28" @default.
• W2014277911 title "In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination" @default.
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• W2014277911 doi "https://doi.org/10.1093/nar/gni175" @default.
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