FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014280130> ?p ?o ?g. }

• W2014280130 endingPage "192" @default.
• W2014280130 startingPage "179" @default.
• W2014280130 abstract "Seven distinct mouse monoclonal antibodies (mAbs) directed against human endothelin-1 (ET-1) have been obtained. On the basis of specificity studies performed with competitive immunoassays and of complementary binding studies, these mAbs were classified in two groups. mAbs of group A (Endo-4, -5, -6 and -10) were shown to be directed against the N terminal loop while those of group B (Endo-2, -8 and -18) recognized the C terminal part of the peptide. A pair of monoclonal antibodies with optimal properties for a two-site immunometric assay were selected and the test was performed in 96-well microtiter plates coated with one mAb (Endo-18), while another mAb (Endo-4) covalently labeled with enzyme acetylcholinesterase was used as tracer. Under optimal conditions, the assay appeared to be very sensitive since concentrations as low as 1 pg/ml could be significantly detected. The precision was also very good with a coefficient of variation below 10% from 3 to 250 pg/ml. The assay was specific for mature endothelin presenting no cross-reactivity with the precursor Big ET-1. On the other hand, strong cross-reactivity was observed with other ET-1-related peptides, including ET-2, ET-3, VIC peptide and sarafotoxin 6-b. The assay permitted specific determination of ET-1 in supernatants of cultured endothelial cells and the validity of the test was demonstrated by HPLC fractionation experiments. In addition, the assay also appeared to be suitable for direct determination of ET-1 in plasma. Studies performed with plasma from healthy subjects revealed that circulating levels of ET-1 are below or close to the detection limit of the method (< 8 pg/ml)." @default.
• W2014280130 created "2016-06-24" @default.
• W2014280130 creator A5013369432 @default.
• W2014280130 creator A5016790368 @default.
• W2014280130 creator A5037097187 @default.
• W2014280130 creator A5037962146 @default.
• W2014280130 creator A5070737798 @default.
• W2014280130 creator A5088588580 @default.
• W2014280130 creator A5089875416 @default.
• W2014280130 date "1993-06-01" @default.
• W2014280130 modified "2023-10-16" @default.
• W2014280130 title "Enzyme immunometric assay for endothelin using tandem monoclonal antibodies" @default.
• W2014280130 cites W1484258981 @default.
• W2014280130 cites W1497952459 @default.
• W2014280130 cites W1516809639 @default.
• W2014280130 cites W1965093875 @default.
• W2014280130 cites W1966329611 @default.
• W2014280130 cites W1971048984 @default.
• W2014280130 cites W1983587158 @default.
• W2014280130 cites W1991775814 @default.
• W2014280130 cites W1993991265 @default.
• W2014280130 cites W1994689045 @default.
• W2014280130 cites W1994869298 @default.
• W2014280130 cites W1995863960 @default.
• W2014280130 cites W2004707896 @default.
• W2014280130 cites W2005385625 @default.
• W2014280130 cites W2009511120 @default.
• W2014280130 cites W2010146641 @default.
• W2014280130 cites W2011508883 @default.
• W2014280130 cites W2013133006 @default.
• W2014280130 cites W2021900417 @default.
• W2014280130 cites W2030072308 @default.
• W2014280130 cites W2030351372 @default.
• W2014280130 cites W2034088331 @default.
• W2014280130 cites W2037224718 @default.
• W2014280130 cites W2038801433 @default.
• W2014280130 cites W2043895418 @default.
• W2014280130 cites W2053283967 @default.
• W2014280130 cites W2060658984 @default.
• W2014280130 cites W2061104442 @default.
• W2014280130 cites W2073968685 @default.
• W2014280130 cites W2076409073 @default.
• W2014280130 cites W2084098131 @default.
• W2014280130 cites W2090072334 @default.
• W2014280130 cites W2100837269 @default.
• W2014280130 cites W2116536722 @default.
• W2014280130 cites W2157603347 @default.
• W2014280130 cites W2162804111 @default.
• W2014280130 cites W2170055655 @default.
• W2014280130 doi "https://doi.org/10.1016/0022-1759(93)90383-i" @default.
• W2014280130 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7686198" @default.
• W2014280130 hasPublicationYear "1993" @default.
• W2014280130 type Work @default.
• W2014280130 sameAs 2014280130 @default.
• W2014280130 citedByCount "26" @default.
• W2014280130 countsByYear W20142801302015 @default.
• W2014280130 crossrefType "journal-article" @default.
• W2014280130 hasAuthorship W2014280130A5013369432 @default.
• W2014280130 hasAuthorship W2014280130A5016790368 @default.
• W2014280130 hasAuthorship W2014280130A5037097187 @default.
• W2014280130 hasAuthorship W2014280130A5037962146 @default.
• W2014280130 hasAuthorship W2014280130A5070737798 @default.
• W2014280130 hasAuthorship W2014280130A5088588580 @default.
• W2014280130 hasAuthorship W2014280130A5089875416 @default.
• W2014280130 hasConcept C144980905 @default.
• W2014280130 hasConcept C153911025 @default.
• W2014280130 hasConcept C159654299 @default.
• W2014280130 hasConcept C163864487 @default.
• W2014280130 hasConcept C170493617 @default.
• W2014280130 hasConcept C181199279 @default.
• W2014280130 hasConcept C185592680 @default.
• W2014280130 hasConcept C203014093 @default.
• W2014280130 hasConcept C2778810118 @default.
• W2014280130 hasConcept C2779281246 @default.
• W2014280130 hasConcept C43617362 @default.
• W2014280130 hasConcept C542903549 @default.
• W2014280130 hasConcept C55493867 @default.
• W2014280130 hasConcept C86803240 @default.
• W2014280130 hasConceptScore W2014280130C144980905 @default.
• W2014280130 hasConceptScore W2014280130C153911025 @default.
• W2014280130 hasConceptScore W2014280130C159654299 @default.
• W2014280130 hasConceptScore W2014280130C163864487 @default.
• W2014280130 hasConceptScore W2014280130C170493617 @default.
• W2014280130 hasConceptScore W2014280130C181199279 @default.
• W2014280130 hasConceptScore W2014280130C185592680 @default.
• W2014280130 hasConceptScore W2014280130C203014093 @default.
• W2014280130 hasConceptScore W2014280130C2778810118 @default.
• W2014280130 hasConceptScore W2014280130C2779281246 @default.
• W2014280130 hasConceptScore W2014280130C43617362 @default.
• W2014280130 hasConceptScore W2014280130C542903549 @default.
• W2014280130 hasConceptScore W2014280130C55493867 @default.
• W2014280130 hasConceptScore W2014280130C86803240 @default.
• W2014280130 hasIssue "2" @default.
• W2014280130 hasLocation W20142801301 @default.
• W2014280130 hasLocation W20142801302 @default.
• W2014280130 hasOpenAccess W2014280130 @default.
• W2014280130 hasPrimaryLocation W20142801301 @default.
• W2014280130 hasRelatedWork W1971294201 @default.

• next