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- W2014284722 abstract "Two alditol acetate methods for the gas chromatographic (GC) analysis of neutral and amino sugars were compared. Following sodium borohydride reduction, one method uses methylimidazole as an acetylation catalyst without prior removal of water or borate salts and the other method uses sodium acetate after removal of borate and water. Depending on the acetylation conditions, muramic acid produced different derivatives. With methylimidazole, reliable derivatization of muramic acid was not possible, although other sugars derivatized reliably. With sodium acetate, all sugars tested were reproducibly derivatized. The utility of the sodium acetate method is shown by the trace GC—mass spectrometric analysis of muramic acid and rhamnose derived from bacterial peptidoglycan-polysaccharide complexes in mammalian tissue." @default.
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- W2014284722 date "1985-01-01" @default.
- W2014284722 modified "2023-09-29" @default.
- W2014284722 title "Modifications in the alditol acetate method for analysis of muramic acid and other neutral and amino sugars by capillary gas chromatography—mass spectrometry with selected ion monitoring" @default.
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- W2014284722 doi "https://doi.org/10.1016/s0021-9673(01)95474-3" @default.
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