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• W2014285753 abstract "We describe a method for construction of hymeric bacteriophage T4 particles displaying foreign polypeptides on their surface. The method is based on our finding that minor T4 fibrous protein fibritin encoded by genewac (whisker's antigen control) could be lengthened at the C terminus without impairing its folding or binding to the phage particle. The lengthened fibritin gene could easily be transferred into the T4 genome by homologous recombination with a plasmid containing the modified genewac. The modified genewac is expressed properly during phage reproduction, and the lengthened fibritin is bound to phage particles. As an example of this type of method, we have obtained the hymeric T4 particles carrying a polypeptide of 53 residues, 45 of which are from the pre-S2 region of hepatitis B virus. The T4 display vector extends currently available display systems." @default.
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• W2014285753 date "1995-06-01" @default.
• W2014285753 modified "2023-10-13" @default.
• W2014285753 title "Bacteriophage T4 as a surface display vector" @default.
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• W2014285753 doi "https://doi.org/10.1007/bf01702598" @default.
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