FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014288051> ?p ?o ?g. }

• W2014288051 endingPage "1487" @default.
• W2014288051 startingPage "1477" @default.
• W2014288051 abstract "Transforming growth factor beta (TGF-beta) regulates the proliferation and differentiation of chondrocytes; however, the mechanism of TGF-beta signal transduction remains unclear. We examined whether the response to TGF-beta is mediated by protein kinase C activity in chondrocytes at different stages of maturation. The aims were to examine the effect of recombinant human TGF-beta 1 (rhTGF-beta 1) on protein kinase C in rat costochondral chondrocyte cultures; determine the major isoform present; assess the involvement of phospholipase C or tyrosine kinases; determine whether genomic or nongenomic pathways are involved; and test whether these mechanisms differ as a function of the stage of cell maturation. Dose-dependent increases in protein kinase C activity were observed in confluent, fourth-passage cultures of rat costochondral growth zone and resting zone chondrocytes treated with rhTGF-beta 1. In growth zone cells, elevated activity was observed at 12 h and decreased markedly by 24 h. In resting zone cells, elevated activity was observed at 9 h, maximum stimulation occurred at 12 h, and activity returned to baseline levels after 48 h. Immunoprecipitation studies showed protein kinase C alpha is the major isoform present in both untreated and treated cells. Neither the phospholipase C inhibitor, U73122, nor the tyrosine kinase inhibitor, genistein, significantly reduced the protein kinase C response to rhTGF-beta 1. Actinomycin D and cycloheximide, inhibitors of transcription and translation, produced dose-dependent inhibition of rhTGF-beta 1 stimulated protein kinase C activity in both resting zone and growth zone chondrocytes. The time course of activation and insensitivity to U73122 suggest that phospholipase C-mediated events are not involved in rhTGF-beta 1 stimulation of protein kinase C in costochondral chondrocytes. Similarly, because genistein had no effect, tyrosine kinases are not implicated. Rather, the reduction in protein kinase C activity observed when rhTGF-beta 1 is administered along with actinomycin D or cycloheximide indicates that new gene expression and protein synthesis are required for the response. These results indicate that the effect of rhTGF-beta 1 is mediated by protein kinase C; however, it is very slow and may require new protein kinase C production, perhaps via a cytokine cascade. Moreover, the classic mechanism of activation of protein kinase C by phospholipase C was not found, suggesting a novel mechanism of activation. Finally, the effects of rhTGF-beta 1 on protein kinase C are dependent on the state of cell maturation with respect to onset and duration of response." @default.
• W2014288051 created "2016-06-24" @default.
• W2014288051 creator A5029880479 @default.
• W2014288051 creator A5038294892 @default.
• W2014288051 creator A5046003294 @default.
• W2014288051 creator A5052368052 @default.
• W2014288051 creator A5067715356 @default.
• W2014288051 creator A5077840467 @default.
• W2014288051 date "2009-12-03" @default.
• W2014288051 modified "2023-10-10" @default.
• W2014288051 title "Regulation of protein kinase C by transforming growth factor β1 in rat costochondral chondrocyte cultures" @default.
• W2014288051 cites W1480727199 @default.
• W2014288051 cites W1487122735 @default.
• W2014288051 cites W1491060698 @default.
• W2014288051 cites W1491958169 @default.
• W2014288051 cites W1505978562 @default.
• W2014288051 cites W1524114372 @default.
• W2014288051 cites W1582355914 @default.
• W2014288051 cites W1595123421 @default.
• W2014288051 cites W1656644300 @default.
• W2014288051 cites W1962334164 @default.
• W2014288051 cites W1965979895 @default.
• W2014288051 cites W1972415345 @default.
• W2014288051 cites W1974747933 @default.
• W2014288051 cites W1975653763 @default.
• W2014288051 cites W1978514667 @default.
• W2014288051 cites W1991310754 @default.
• W2014288051 cites W1992172901 @default.
• W2014288051 cites W1994190823 @default.
• W2014288051 cites W1994552438 @default.
• W2014288051 cites W1996716065 @default.
• W2014288051 cites W1999566158 @default.
• W2014288051 cites W2006893504 @default.
• W2014288051 cites W2007249705 @default.
• W2014288051 cites W2008407614 @default.
• W2014288051 cites W2009915497 @default.
• W2014288051 cites W2016068011 @default.
• W2014288051 cites W2021218223 @default.
• W2014288051 cites W2022189167 @default.
• W2014288051 cites W2025194786 @default.
• W2014288051 cites W2030464593 @default.
• W2014288051 cites W2040287483 @default.
• W2014288051 cites W2042187005 @default.
• W2014288051 cites W2046694960 @default.
• W2014288051 cites W2048868013 @default.
• W2014288051 cites W2052982824 @default.
• W2014288051 cites W2072273255 @default.
• W2014288051 cites W2080987866 @default.
• W2014288051 cites W2094780893 @default.
• W2014288051 cites W2095318593 @default.
• W2014288051 cites W2104433443 @default.
• W2014288051 cites W2108104357 @default.
• W2014288051 cites W2136376840 @default.
• W2014288051 cites W2136819827 @default.
• W2014288051 cites W2160176627 @default.
• W2014288051 cites W2171094619 @default.
• W2014288051 cites W2228774691 @default.
• W2014288051 cites W2335961456 @default.
• W2014288051 cites W4232534952 @default.
• W2014288051 doi "https://doi.org/10.1002/jbmr.5650090921" @default.
• W2014288051 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7817833" @default.
• W2014288051 hasPublicationYear "2009" @default.
• W2014288051 type Work @default.
• W2014288051 sameAs 2014288051 @default.
• W2014288051 citedByCount "25" @default.
• W2014288051 crossrefType "journal-article" @default.
• W2014288051 hasAuthorship W2014288051A5029880479 @default.
• W2014288051 hasAuthorship W2014288051A5038294892 @default.
• W2014288051 hasAuthorship W2014288051A5046003294 @default.
• W2014288051 hasAuthorship W2014288051A5052368052 @default.
• W2014288051 hasAuthorship W2014288051A5067715356 @default.
• W2014288051 hasAuthorship W2014288051A5077840467 @default.
• W2014288051 hasConcept C126322002 @default.
• W2014288051 hasConcept C134018914 @default.
• W2014288051 hasConcept C153911025 @default.
• W2014288051 hasConcept C184235292 @default.
• W2014288051 hasConcept C195794163 @default.
• W2014288051 hasConcept C202751555 @default.
• W2014288051 hasConcept C2778128677 @default.
• W2014288051 hasConcept C2778229498 @default.
• W2014288051 hasConcept C2781403057 @default.
• W2014288051 hasConcept C3675279 @default.
• W2014288051 hasConcept C42362537 @default.
• W2014288051 hasConcept C55493867 @default.
• W2014288051 hasConcept C62478195 @default.
• W2014288051 hasConcept C71924100 @default.
• W2014288051 hasConcept C86803240 @default.
• W2014288051 hasConcept C95444343 @default.
• W2014288051 hasConcept C97029542 @default.
• W2014288051 hasConceptScore W2014288051C126322002 @default.
• W2014288051 hasConceptScore W2014288051C134018914 @default.
• W2014288051 hasConceptScore W2014288051C153911025 @default.
• W2014288051 hasConceptScore W2014288051C184235292 @default.
• W2014288051 hasConceptScore W2014288051C195794163 @default.
• W2014288051 hasConceptScore W2014288051C202751555 @default.
• W2014288051 hasConceptScore W2014288051C2778128677 @default.
• W2014288051 hasConceptScore W2014288051C2778229498 @default.
• W2014288051 hasConceptScore W2014288051C2781403057 @default.

• next