FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014294474> ?p ?o ?g. }

• W2014294474 endingPage "188" @default.
• W2014294474 startingPage "182" @default.
• W2014294474 abstract "Isotope fractionation is a powerful technique by which to probe the reaction mechanism of enzymes. The effect of a heavy isotope on the reaction energetics can be used to predict transition state architecture and reaction mechanism. In order to examine simultaneously the isotope fractionation in 13C at multiple sites within the substrate and product molecules without any need for site-selective isotope enrichment, a technique exploiting quantitative isotopic nuclear magnetic resonance (NMR) spectrometry at natural abundance (NAQ–NMR) has been developed. Here we report the first application of this technique to the study of an enzyme-catalyzed reaction, the bioconversion of ferulic acid to vanillin in cultures of Streptomyces setonii. We were able to show that the NAQ–NMR methodology is sufficiently precise and robust to measure the isotope shifts in the 13C/12C ratios in both substrate and product of this biotransformation, thereby permitting meaningful data to be obtained even at carbon positions that take part only indirectly in the reaction and show only secondary isotope fractionation. The results obtained provide direct evidence in support of the current hypothesis for the reaction mechanism of the enzyme hydroxycinnamoyl–CoA hydratase/lyase, notably the proposed involvement of the quinone methide enolate of feruloyl–CoA as intermediate in the catalytic pathway." @default.
• W2014294474 created "2016-06-24" @default.
• W2014294474 creator A5016771967 @default.
• W2014294474 creator A5027331101 @default.
• W2014294474 creator A5035748063 @default.
• W2014294474 creator A5044163447 @default.
• W2014294474 creator A5048922604 @default.
• W2014294474 creator A5060607658 @default.
• W2014294474 creator A5081759257 @default.
• W2014294474 date "2009-10-01" @default.
• W2014294474 modified "2023-09-30" @default.
• W2014294474 title "Quantitative isotopic 13C nuclear magnetic resonance at natural abundance to probe enzyme reaction mechanisms via site-specific isotope fractionation: The case of the chain-shortening reaction for the bioconversion of ferulic acid to vanillin" @default.
• W2014294474 cites W1581694888 @default.
• W2014294474 cites W1856682990 @default.
• W2014294474 cites W1967316786 @default.
• W2014294474 cites W1983026200 @default.
• W2014294474 cites W1985614553 @default.
• W2014294474 cites W1989632349 @default.
• W2014294474 cites W1991805990 @default.
• W2014294474 cites W1999581086 @default.
• W2014294474 cites W2002017096 @default.
• W2014294474 cites W2005968905 @default.
• W2014294474 cites W2019087145 @default.
• W2014294474 cites W2020029159 @default.
• W2014294474 cites W2024678623 @default.
• W2014294474 cites W2028521189 @default.
• W2014294474 cites W2035539862 @default.
• W2014294474 cites W2056423487 @default.
• W2014294474 cites W2061126917 @default.
• W2014294474 cites W2076772062 @default.
• W2014294474 cites W2076773608 @default.
• W2014294474 cites W2078981916 @default.
• W2014294474 cites W2084506565 @default.
• W2014294474 cites W2087471660 @default.
• W2014294474 cites W2098261952 @default.
• W2014294474 cites W2107695185 @default.
• W2014294474 cites W2122544754 @default.
• W2014294474 cites W2153634914 @default.
• W2014294474 cites W2157642804 @default.
• W2014294474 doi "https://doi.org/10.1016/j.ab.2009.06.031" @default.
• W2014294474 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19563771" @default.
• W2014294474 hasPublicationYear "2009" @default.
• W2014294474 type Work @default.
• W2014294474 sameAs 2014294474 @default.
• W2014294474 citedByCount "25" @default.
• W2014294474 countsByYear W20142944742012 @default.
• W2014294474 countsByYear W20142944742013 @default.
• W2014294474 countsByYear W20142944742014 @default.
• W2014294474 countsByYear W20142944742015 @default.
• W2014294474 countsByYear W20142944742016 @default.
• W2014294474 countsByYear W20142944742017 @default.
• W2014294474 countsByYear W20142944742018 @default.
• W2014294474 countsByYear W20142944742020 @default.
• W2014294474 countsByYear W20142944742022 @default.
• W2014294474 crossrefType "journal-article" @default.
• W2014294474 hasAuthorship W2014294474A5016771967 @default.
• W2014294474 hasAuthorship W2014294474A5027331101 @default.
• W2014294474 hasAuthorship W2014294474A5035748063 @default.
• W2014294474 hasAuthorship W2014294474A5044163447 @default.
• W2014294474 hasAuthorship W2014294474A5048922604 @default.
• W2014294474 hasAuthorship W2014294474A5060607658 @default.
• W2014294474 hasAuthorship W2014294474A5081759257 @default.
• W2014294474 hasConcept C100544194 @default.
• W2014294474 hasConcept C111368507 @default.
• W2014294474 hasConcept C121332964 @default.
• W2014294474 hasConcept C127313418 @default.
• W2014294474 hasConcept C164304813 @default.
• W2014294474 hasConcept C178790620 @default.
• W2014294474 hasConcept C185592680 @default.
• W2014294474 hasConcept C2777289219 @default.
• W2014294474 hasConcept C2779334269 @default.
• W2014294474 hasConcept C2780074830 @default.
• W2014294474 hasConcept C2780213334 @default.
• W2014294474 hasConcept C36674120 @default.
• W2014294474 hasConcept C43617362 @default.
• W2014294474 hasConcept C58364064 @default.
• W2014294474 hasConcept C62520636 @default.
• W2014294474 hasConcept C69928629 @default.
• W2014294474 hasConcept C97428945 @default.
• W2014294474 hasConceptScore W2014294474C100544194 @default.
• W2014294474 hasConceptScore W2014294474C111368507 @default.
• W2014294474 hasConceptScore W2014294474C121332964 @default.
• W2014294474 hasConceptScore W2014294474C127313418 @default.
• W2014294474 hasConceptScore W2014294474C164304813 @default.
• W2014294474 hasConceptScore W2014294474C178790620 @default.
• W2014294474 hasConceptScore W2014294474C185592680 @default.
• W2014294474 hasConceptScore W2014294474C2777289219 @default.
• W2014294474 hasConceptScore W2014294474C2779334269 @default.
• W2014294474 hasConceptScore W2014294474C2780074830 @default.
• W2014294474 hasConceptScore W2014294474C2780213334 @default.
• W2014294474 hasConceptScore W2014294474C36674120 @default.
• W2014294474 hasConceptScore W2014294474C43617362 @default.
• W2014294474 hasConceptScore W2014294474C58364064 @default.
• W2014294474 hasConceptScore W2014294474C62520636 @default.
• W2014294474 hasConceptScore W2014294474C69928629 @default.
• W2014294474 hasConceptScore W2014294474C97428945 @default.
• W2014294474 hasIssue "2" @default.
• W2014294474 hasLocation W20142944741 @default.

• next