Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014305757> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2014305757 endingPage "472" @default.
- W2014305757 startingPage "467" @default.
- W2014305757 abstract "In this work, a papain inhibitor extracted from seeds of Adenanthera pavonina was covalently immobilized onto glutaraldehyde modified polyaniline (PANIG). The immobilization was very efficient, presenting 54.24% inhibitor retention in the following conditions: immobilization time of 30 min, at 4 °C, pH 7.0, inhibitor concentration of 20 mg and 5 mg of PANIG. The resultant material (PANIG-I) was tested as stationary phase for the cysteine proteases papain, ficin and bromelain purification through bioaffinity chromatography. A commercial preparation of papain was efficiently purified, resulting in a single band with 25 kDa. SDS–PAGE of purified bromelain showed a characteristic band around 28 kDa and this procedure resulted in 0.89-fold purification and 32.4% yield. The purification process was more effective for ficin reaching 2.6-fold purification and a single band of 25 kDa in the SDS–PAGE. These results showed that the stationary phase containing A. pavonina inhibitors immobilized onto PANIG is a very promising material for a single step purification of cysteine proteases through bioaffinity chromatography." @default.
- W2014305757 created "2016-06-24" @default.
- W2014305757 creator A5007753667 @default.
- W2014305757 creator A5032958857 @default.
- W2014305757 creator A5037338922 @default.
- W2014305757 creator A5048694664 @default.
- W2014305757 date "2013-12-01" @default.
- W2014305757 modified "2023-10-11" @default.
- W2014305757 title "The use of papain inhibitor immobilized onto polyaniline for bioaffinity chromatography of cysteine proteases" @default.
- W2014305757 cites W1963808575 @default.
- W2014305757 cites W1967857459 @default.
- W2014305757 cites W1982230721 @default.
- W2014305757 cites W1988498979 @default.
- W2014305757 cites W1989852193 @default.
- W2014305757 cites W2003349260 @default.
- W2014305757 cites W2013044355 @default.
- W2014305757 cites W2014179519 @default.
- W2014305757 cites W2018211654 @default.
- W2014305757 cites W2022264364 @default.
- W2014305757 cites W2024408399 @default.
- W2014305757 cites W2033515425 @default.
- W2014305757 cites W2042647021 @default.
- W2014305757 cites W2044736244 @default.
- W2014305757 cites W2044853699 @default.
- W2014305757 cites W2049898908 @default.
- W2014305757 cites W2054139078 @default.
- W2014305757 cites W2058645846 @default.
- W2014305757 cites W2059442894 @default.
- W2014305757 cites W2068841951 @default.
- W2014305757 cites W2072887184 @default.
- W2014305757 cites W2079017871 @default.
- W2014305757 cites W2089662948 @default.
- W2014305757 cites W2100837269 @default.
- W2014305757 cites W2128635872 @default.
- W2014305757 doi "https://doi.org/10.1016/j.seppur.2013.10.027" @default.
- W2014305757 hasPublicationYear "2013" @default.
- W2014305757 type Work @default.
- W2014305757 sameAs 2014305757 @default.
- W2014305757 citedByCount "3" @default.
- W2014305757 countsByYear W20143057572016 @default.
- W2014305757 countsByYear W20143057572017 @default.
- W2014305757 countsByYear W20143057572018 @default.
- W2014305757 crossrefType "journal-article" @default.
- W2014305757 hasAuthorship W2014305757A5007753667 @default.
- W2014305757 hasAuthorship W2014305757A5032958857 @default.
- W2014305757 hasAuthorship W2014305757A5037338922 @default.
- W2014305757 hasAuthorship W2014305757A5048694664 @default.
- W2014305757 hasConcept C178790620 @default.
- W2014305757 hasConcept C180577832 @default.
- W2014305757 hasConcept C181199279 @default.
- W2014305757 hasConcept C182220744 @default.
- W2014305757 hasConcept C185592680 @default.
- W2014305757 hasConcept C2777334355 @default.
- W2014305757 hasConcept C2777529624 @default.
- W2014305757 hasConcept C2779201268 @default.
- W2014305757 hasConcept C2779832904 @default.
- W2014305757 hasConcept C43617362 @default.
- W2014305757 hasConcept C55493867 @default.
- W2014305757 hasConceptScore W2014305757C178790620 @default.
- W2014305757 hasConceptScore W2014305757C180577832 @default.
- W2014305757 hasConceptScore W2014305757C181199279 @default.
- W2014305757 hasConceptScore W2014305757C182220744 @default.
- W2014305757 hasConceptScore W2014305757C185592680 @default.
- W2014305757 hasConceptScore W2014305757C2777334355 @default.
- W2014305757 hasConceptScore W2014305757C2777529624 @default.
- W2014305757 hasConceptScore W2014305757C2779201268 @default.
- W2014305757 hasConceptScore W2014305757C2779832904 @default.
- W2014305757 hasConceptScore W2014305757C43617362 @default.
- W2014305757 hasConceptScore W2014305757C55493867 @default.
- W2014305757 hasLocation W20143057571 @default.
- W2014305757 hasOpenAccess W2014305757 @default.
- W2014305757 hasPrimaryLocation W20143057571 @default.
- W2014305757 hasRelatedWork W176482322 @default.
- W2014305757 hasRelatedWork W2009392789 @default.
- W2014305757 hasRelatedWork W2037301053 @default.
- W2014305757 hasRelatedWork W2039911253 @default.
- W2014305757 hasRelatedWork W2093154896 @default.
- W2014305757 hasRelatedWork W2387268001 @default.
- W2014305757 hasRelatedWork W2629048911 @default.
- W2014305757 hasRelatedWork W2983135087 @default.
- W2014305757 hasRelatedWork W3112440513 @default.
- W2014305757 hasRelatedWork W4224443410 @default.
- W2014305757 hasVolume "120" @default.
- W2014305757 isParatext "false" @default.
- W2014305757 isRetracted "false" @default.
- W2014305757 magId "2014305757" @default.
- W2014305757 workType "article" @default.