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- W2014309610 abstract "We developed a new system for detection of whole-genome differentiation using DNA-DNA hybridization, and tested its sensitivity with three closely-related Fusarium species. We compared DNA-DNA relatedness to nucleotide sequence homologies of five genetic regions between each of five strains of three Fusarium species. DNA-DNA relatedness by our system was 16.2–86.6%. Sequence homologies of 18S rDNA, rDNA cluster region from ITS1 to 28S rDNA, β-tub, EF-1α and lys2 were 100.0, 99.0–100.0, 96.7–100.0, 95.1–99.4, and 94.7–100.0%, respectively. Our system could clearly detect differentiation between closely-related fungal species which have very similar morphological-characteristics, and exhibit little diagnoses in nucleotide sequences. Our results suggest that this system is a good tool for identification and phylogenetic analysis of closely-related fungal species." @default.
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- W2014309610 date "2012-01-01" @default.
- W2014309610 modified "2023-09-26" @default.
- W2014309610 title "Sensitive Detection of Whole-Genome Differentiation among Closely-Related Species of the Genus <i>Fusarium</i> Using DNA-DNA Hybridization and a Microplate Technique" @default.
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- W2014309610 doi "https://doi.org/10.1292/jvms.12-0034" @default.
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