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• W2014309938 abstract "Selenoproteins result from the incorporation of selenocysteine (Sec-U) at an UGA-stop codon positioned within a gene's open reading frame and directed by selenocysteine insertion sequence (SECIS) elements. Although the selenocysteine incorporation pathway has been identified in a wide range of organisms it has not yet been reported in the Kinetoplastida Leishmania and Trypanosoma. Here we present evidence consistent with the presence of a selenocysteine biosynthetic pathway in Kinetoplastida. These include the existence of SECIS-containing coding sequences in Leishmania major and Leishmania infantum, the incorporation of 75Se into Leishmania proteins, the occurrence of selenocysteine-tRNA (tRNAucasec) in both Leishmania and Trypanosoma and in addition the finding of all genes necessary for selenocysteine synthesis such as SELB, SELD, PSTK and SECp43. As in other eukaryotes, the Kinetoplastids have no identifiable SELA homologue. To our knowledge this is the first report on the identification of selenocysteine insertion machinery in Kinetoplastida, more specifically in Leishmania, at the sequence level." @default.
• W2014309938 created "2016-06-24" @default.
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• W2014309938 date "2006-10-01" @default.
• W2014309938 modified "2023-10-03" @default.
• W2014309938 title "Identification of Leishmania selenoproteins and SECIS element" @default.
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• W2014309938 doi "https://doi.org/10.1016/j.molbiopara.2006.05.002" @default.
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