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• W2014310505 abstract "ABSTRACT In comparison to other pseudomonads, Pseudomonas aeruginosa grows poorly in l -lysine as a sole source of nutrient. In this study, the ldcA gene ( l ysine d e c arboxylase A ; PA1818), previously identified as a member of the ArgR regulon of l -arginine metabolism, was found essential for l -lysine catabolism in this organism. LdcA was purified to homogeneity from a recombinant strain of Escherichia coli , and the results of enzyme characterization revealed that this pyridoxal-5-phosphate-dependent decarboxylase takes l -lysine, but not l -arginine, as a substrate. At an optimal pH of 8.5, cooperative substrate activation by l -lysine was depicted from kinetics studies, with calculated K m and V max values of 0.73 mM and 2.2 μmole/mg/min, respectively. Contrarily, the ldcA promoter was induced by exogenous l -arginine but not by l -lysine in the wild-type strain PAO1, and the binding of ArgR to this promoter region was demonstrated by electromobility shift assays. This peculiar arginine control on lysine utilization was also noted from uptake experiments in which incorporation of radioactively labeled l -lysine was enhanced in cells grown in the presence of l -arginine but not l -lysine. Rapid growth on l -lysine was detected in a mutant devoid of the main arginine catabolic pathway and with a higher basal level of the intracellular l -arginine pool and hence elevated ArgR-responsive regulons, including ldcA . Growth on l -lysine as a nitrogen source can also be enhanced when the aruH gene encoding an arginine/lysine:pyruvate transaminase was expressed constitutively from plasmids; however, no growth of the ldcA mutant on l -lysine suggests a minor role of this transaminase in l -lysine catabolism. In summary, this study reveals a tight connection of lysine catabolism to the arginine regulatory network, and the lack of lysine-responsive control on lysine uptake and decarboxylation provides an explanation of l -lysine as a poor nutrient for P. aeruginosa ." @default.
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• W2014310505 date "2010-11-15" @default.
• W2014310505 modified "2023-09-26" @default.
• W2014310505 title "<scp>l</scp> -Lysine Catabolism Is Controlled by <scp>l</scp> -Arginine and ArgR in <i>Pseudomonas aeruginosa</i> PAO1" @default.
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• W2014310505 doi "https://doi.org/10.1128/jb.00673-10" @default.
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