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• W2014370108 abstract "La identificación proteómica de micobacterias no tuberculosas (MNTs) mediante MALDI-TOF presenta una mayor complejidad debido a la especial composición de su pared celular, que complica la extracción de proteínas. Un total de 106 aislamientos pertenecientes a diferentes especies de MNTs procedentes de muestras clínicas del Complejo Asistencial Universitario de León recogidas durante los años 2019 y 2020 se han identificado por un método proteómico abreviado (MALDI-TOF Biotyper Bruker®) desarrollado en nuestro laboratorio. La identificación se ha comparado con la realizada en paralelo en el Centro de Referencia de Majadahonda. Se analizaron un total de 22 especies diferentes de MNTs obteniendo una concordancia del 91,5%. Las nueve discrepancias detectadas se dieron entre especies pertenecientes al mismo grupo taxonómico. En el 67,92% de las identificaciones el score fue superior a 1,8. En el tiempo de procesamiento se obtuvo un ahorro aproximado de 24 minutos con respecto al recomendado por el fabricante.Proteomic techniques relaying upon mass spectrometry (MALDI_TOF) applied to nontuberculous mycobacteria (NTM) identification, constitute a difficult goal. Cell wall structure features complicates the protein extraction procedure. A total of 106 isolates belonging to a variety of MNTs species isolated from clinical samples taken at the Complejo Asistencial Universitario de León for a two years period (2019-20) were identified following a simplified method (MALDI-TOF Biotyper Bruker®) developped in our laboratory. The resultant identification was compared to a parallel one ruled on the Centro de Referencia de Majadahonda. A total of 22 different MNTs species were tested, obtaining an agreement of 91,5%. Only 9 minor discrepancies between species belonging to the same taxonomic group of MNTs were detected. The score obtained in the 67.92% of the cases was higher than 1.8. A time-saving of 24 minutes compared to the manufacturer‘s proceeding was achieved." @default.
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• W2014370108 date "2013-09-01" @default.
• W2014370108 modified "2023-10-16" @default.
• W2014370108 title "Infección diseminada por micobacterias no tuberculosas en paciente con síndrome hiper-IgE" @default.
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• W2014370108 doi "https://doi.org/10.1016/j.anpedi.2013.01.017" @default.
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