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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014374956> ?p ?o ?g. }

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• W2014374956 abstract "A highly sensitive and specific two-dimensional electron-capture gas chromatographic method has been developed for determining small amounts of DNA by measuring its thymine content. The method can also be used to measure RNA based on uracil content. The nucleic acids were hydrolyzed and released constituents were separated and detected as their chloromethyldimethylsilyl ethers. The minimal amount detected was 5 pg of each base. Standard curves were linear from 5 to 200 pg. This method allowed quantitative determination of 2 ng of DNA (routinely detectable quantity) after hydrolysis of biological material in formic acid, even in the presence of large amounts of RNA and/or protein. For example, this method has been shown to be successful in determination of the DNA contents of manually isolated nucleic such as from amphibian oocytes. Besides being accurate, the procedure was rapid: after maximal hydrolysis (usually about 45 min) the derivatization and gas chromatographic analysis was completed in another 15 min. The procedure described represents a direct biochemical alternative to cytophotometric estimation of nuclear contents and has the advantage of providing values for absolute DNA content per nucleus." @default.
• W2014374956 created "2016-06-24" @default.
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• W2014374956 date "1978-06-01" @default.
• W2014374956 modified "2023-09-26" @default.
• W2014374956 title "Quantitative microdetermination of DNA by two-dimensional electron capture gas chromatography of the thymine constituent" @default.
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• W2014374956 doi "https://doi.org/10.1016/0003-2697(78)90772-8" @default.
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