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- W2014380055 abstract "A new method for quantifying class-specific antibodies is presented. The method has been named Diffusion-In-Gel-Enzyme-Linked-ImmunoSorbentAssay (DIG-ELISA), and is briefly as follows. Antiserum ia allowed to diffuse from wells in a gel layered over an antigen-coated plastic surface. The gel is then removed and the preparation is incubated with enzyme-conjugated anti-immunoglobulin. The enzyme is then visualised in situ by a colour reaction produced by pouring a substrate-containing gel over the plastic surface. Bovine serum albumin and rabbit-anti-BSA were used as a model system, and horseradish peroxidase or alkaline phosphatase as enzymes for visualization." @default.
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- W2014380055 date "1979-12-01" @default.
- W2014380055 modified "2023-10-17" @default.
- W2014380055 title "Diffusion in gel-enzyme linked immunosorbent assay (DIG-ELISA): A simple method for quantitation of class-specific antibodies" @default.
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- W2014380055 doi "https://doi.org/10.1016/0022-1759(79)90290-4" @default.
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