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• W2014394269 abstract "The pathogenesis of the primary tissue degeneration that limits the life-span of aortic and pulmonary homografts has still not been revealed. Histopathological studies on homograft explants have not given definitive insight into the eventual fate of donor cells, nor have they demonstrated the assumed importance of host cell ingrowth into the graft tissue. In this experimental study, fluorescence in situ hybridization (FISH) is introduced as a new approach to examine the distribution of host and donor cells in homograft explants. Aortic valve replacement was performed with a cryopreserved porcine aortic homograft in three pigs; donor and recipient were of opposite sex. After 4 months, the grafts were explanted and examined by FISH using a biotinylated porcine Y-chromosome-specific library probe. Following probe detection with FITC-conjugated avidin, a clear distinction could be made between cells of host and donor origin without distorting the histological integrity of the explants. There was ingrowth of donor cells into the graft aortic wall and into the valve leaflet, to some extent. In all explants, remaining donor cells were present, though decreased in number. The introduction of FISH in homograft heart valve research provides a powerful tool to study the fate of recipient and donor cellular elements in situ, and may therefore contribute to a better understanding of the histopathological processes that take place in transplanted homograft valves." @default.
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• W2014394269 date "1997-09-01" @default.
• W2014394269 modified "2023-10-16" @default.
• W2014394269 title "Identification of host and donor cells in porcine homograft heart valve explants by fluorescencein situ hybridization" @default.
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• W2014394269 doi "https://doi.org/10.1002/(sici)1096-9896(199709)183:1<99::aid-path1086>3.0.co;2-l" @default.
• W2014394269 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9370954" @default.
• W2014394269 hasPublicationYear "1997" @default.
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