FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014426503> ?p ?o ?g. }

• W2014426503 endingPage "3051" @default.
• W2014426503 startingPage "3044" @default.
• W2014426503 abstract "ABSTRACT Assigning functions to newly discovered genes constitutes one of the major challenges en route to fully exploiting the data becoming available from the genome sequencing initiatives. Heterologous expression in an appropriate host is central in functional genomics studies. In this context, filamentous fungi offer many advantages over bacterial and yeast systems. To facilitate the use of filamentous fungi in functional genomics, we present a versatile cloning system that allows a gene of interest to be expressed from a defined genomic location of Aspergillus nidulans . By a single USER cloning step, genes are easily inserted into a combined targeting-expression cassette ready for rapid integration and analysis. The system comprises a vector set that allows genes to be expressed either from the constitutive PgpdA promoter or from the inducible PalcA promoter. Moreover, by using the vector set, protein variants can easily be made and expressed from the same locus, which is mandatory for proper comparative analyses. Lastly, all individual elements of the vectors can easily be substituted for other similar elements, ensuring the flexibility of the system. We have demonstrated the potential of the system by transferring the 7,745-bp large mpaC gene from Penicillium brevicompactum to A. nidulans . In parallel, we produced defined mutant derivatives of mpaC , and the combined analysis of A. nidulans strains expressing mpaC or mutated mpaC genes unequivocally demonstrated that mpaC indeed encodes a polyketide synthase that produces the first intermediate in the production of the medically important immunosuppressant mycophenolic acid." @default.
• W2014426503 created "2016-06-24" @default.
• W2014426503 creator A5003040829 @default.
• W2014426503 creator A5003491281 @default.
• W2014426503 creator A5005626106 @default.
• W2014426503 creator A5010212537 @default.
• W2014426503 creator A5012388749 @default.
• W2014426503 creator A5020329649 @default.
• W2014426503 creator A5054962508 @default.
• W2014426503 creator A5083316331 @default.
• W2014426503 creator A5084611041 @default.
• W2014426503 creator A5086722753 @default.
• W2014426503 date "2011-05-01" @default.
• W2014426503 modified "2023-10-12" @default.
• W2014426503 title "Versatile Enzyme Expression and Characterization System for Aspergillus nidulans, with the Penicillium brevicompactum Polyketide Synthase Gene from the Mycophenolic Acid Gene Cluster as a Test Case" @default.
• W2014426503 cites W1487041098 @default.
• W2014426503 cites W170364932 @default.
• W2014426503 cites W1964727634 @default.
• W2014426503 cites W1966501206 @default.
• W2014426503 cites W1990621556 @default.
• W2014426503 cites W2008685087 @default.
• W2014426503 cites W2015856203 @default.
• W2014426503 cites W2021214912 @default.
• W2014426503 cites W2025748246 @default.
• W2014426503 cites W2032269112 @default.
• W2014426503 cites W2043200125 @default.
• W2014426503 cites W2049627092 @default.
• W2014426503 cites W2055771781 @default.
• W2014426503 cites W2061057323 @default.
• W2014426503 cites W2063740246 @default.
• W2014426503 cites W2068116975 @default.
• W2014426503 cites W2075889739 @default.
• W2014426503 cites W2082605184 @default.
• W2014426503 cites W2086118349 @default.
• W2014426503 cites W2121980868 @default.
• W2014426503 cites W2127936340 @default.
• W2014426503 cites W2138888519 @default.
• W2014426503 cites W2139841201 @default.
• W2014426503 cites W2145115293 @default.
• W2014426503 cites W2147263983 @default.
• W2014426503 cites W2161645265 @default.
• W2014426503 cites W2169180708 @default.
• W2014426503 doi "https://doi.org/10.1128/aem.01768-10" @default.
• W2014426503 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3126399" @default.
• W2014426503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21398493" @default.
• W2014426503 hasPublicationYear "2011" @default.
• W2014426503 type Work @default.
• W2014426503 sameAs 2014426503 @default.
• W2014426503 citedByCount "87" @default.
• W2014426503 countsByYear W20144265032012 @default.
• W2014426503 countsByYear W20144265032013 @default.
• W2014426503 countsByYear W20144265032014 @default.
• W2014426503 countsByYear W20144265032015 @default.
• W2014426503 countsByYear W20144265032016 @default.
• W2014426503 countsByYear W20144265032017 @default.
• W2014426503 countsByYear W20144265032018 @default.
• W2014426503 countsByYear W20144265032019 @default.
• W2014426503 countsByYear W20144265032020 @default.
• W2014426503 countsByYear W20144265032021 @default.
• W2014426503 countsByYear W20144265032022 @default.
• W2014426503 countsByYear W20144265032023 @default.
• W2014426503 crossrefType "journal-article" @default.
• W2014426503 hasAuthorship W2014426503A5003040829 @default.
• W2014426503 hasAuthorship W2014426503A5003491281 @default.
• W2014426503 hasAuthorship W2014426503A5005626106 @default.
• W2014426503 hasAuthorship W2014426503A5010212537 @default.
• W2014426503 hasAuthorship W2014426503A5012388749 @default.
• W2014426503 hasAuthorship W2014426503A5020329649 @default.
• W2014426503 hasAuthorship W2014426503A5054962508 @default.
• W2014426503 hasAuthorship W2014426503A5083316331 @default.
• W2014426503 hasAuthorship W2014426503A5084611041 @default.
• W2014426503 hasAuthorship W2014426503A5086722753 @default.
• W2014426503 hasBestOaLocation W20144265031 @default.
• W2014426503 hasConcept C104317684 @default.
• W2014426503 hasConcept C141231307 @default.
• W2014426503 hasConcept C143065580 @default.
• W2014426503 hasConcept C161078062 @default.
• W2014426503 hasConcept C189206191 @default.
• W2014426503 hasConcept C2776923806 @default.
• W2014426503 hasConcept C2777763344 @default.
• W2014426503 hasConcept C2778635478 @default.
• W2014426503 hasConcept C36857842 @default.
• W2014426503 hasConcept C54355233 @default.
• W2014426503 hasConcept C553450214 @default.
• W2014426503 hasConcept C70721500 @default.
• W2014426503 hasConcept C86803240 @default.
• W2014426503 hasConceptScore W2014426503C104317684 @default.
• W2014426503 hasConceptScore W2014426503C141231307 @default.
• W2014426503 hasConceptScore W2014426503C143065580 @default.
• W2014426503 hasConceptScore W2014426503C161078062 @default.
• W2014426503 hasConceptScore W2014426503C189206191 @default.
• W2014426503 hasConceptScore W2014426503C2776923806 @default.
• W2014426503 hasConceptScore W2014426503C2777763344 @default.
• W2014426503 hasConceptScore W2014426503C2778635478 @default.
• W2014426503 hasConceptScore W2014426503C36857842 @default.
• W2014426503 hasConceptScore W2014426503C54355233 @default.
• W2014426503 hasConceptScore W2014426503C553450214 @default.
• W2014426503 hasConceptScore W2014426503C70721500 @default.

• next