FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014451608> ?p ?o ?g. }

• W2014451608 abstract "Plant leaves are optically complex, which makes them difficult to image by light microscopy. Careful sample preparation is therefore required to enable researchers to maximize the information gained from advances in fluorescent protein labeling, cell dyes and innovations in microscope technologies and techniques. We have previously shown that mounting leaves in the non-toxic, non-fluorescent perfluorocarbon (PFC), perfluorodecalin (PFD) enhances the optical properties of the leaf with minimal impact on physiology. Here, we assess the use of the PFCs, PFD, and perfluoroperhydrophenanthrene (PP11) for in vivo plant leaf imaging using four advanced modes of microscopy: laser scanning confocal microscopy (LSCM), two-photon fluorescence microscopy, second harmonic generation microscopy, and stimulated Raman scattering (SRS) microscopy. For every mode of imaging tested, we observed an improved signal when leaves were mounted in PFD or in PP11, compared to mounting the samples in water. Using an image analysis technique based on autocorrelation to quantitatively assess LSCM image deterioration with depth, we show that PP11 outperformed PFD as a mounting medium by enabling the acquisition of clearer images deeper into the tissue. In addition, we show that SRS microscopy can be used to image PFCs directly in the mesophyll and thereby easily delimit the negative space within a leaf, which may have important implications for studies of leaf development. Direct comparison of on and off resonance SRS micrographs show that PFCs do not to form intracellular aggregates in live plants. We conclude that the application of PFCs as mounting media substantially increases advanced microscopy image quality of living mesophyll and leaf vascular bundle cells." @default.
• W2014451608 created "2016-06-24" @default.
• W2014451608 creator A5003362374 @default.
• W2014451608 creator A5011671405 @default.
• W2014451608 creator A5016900158 @default.
• W2014451608 creator A5023119806 @default.
• W2014451608 creator A5026839832 @default.
• W2014451608 creator A5037813372 @default.
• W2014451608 creator A5044369215 @default.
• W2014451608 creator A5064805550 @default.
• W2014451608 creator A5066038343 @default.
• W2014451608 creator A5086504077 @default.
• W2014451608 date "2014-04-23" @default.
• W2014451608 modified "2023-10-06" @default.
• W2014451608 title "An update: improvements in imaging perfluorocarbon-mounted plant leaves with implications for studies of plant pathology, physiology, development and cell biology" @default.
• W2014451608 cites W1487375396 @default.
• W2014451608 cites W1968358148 @default.
• W2014451608 cites W1971023078 @default.
• W2014451608 cites W2006118574 @default.
• W2014451608 cites W2008971218 @default.
• W2014451608 cites W2017241202 @default.
• W2014451608 cites W2030802738 @default.
• W2014451608 cites W2030975955 @default.
• W2014451608 cites W2033843824 @default.
• W2014451608 cites W2036375664 @default.
• W2014451608 cites W2039210066 @default.
• W2014451608 cites W2041434827 @default.
• W2014451608 cites W2044465660 @default.
• W2014451608 cites W2045901799 @default.
• W2014451608 cites W2051128904 @default.
• W2014451608 cites W2064409884 @default.
• W2014451608 cites W2101545913 @default.
• W2014451608 cites W2101859816 @default.
• W2014451608 cites W2109027740 @default.
• W2014451608 cites W2115318086 @default.
• W2014451608 cites W2121928615 @default.
• W2014451608 cites W2128242032 @default.
• W2014451608 cites W2147122730 @default.
• W2014451608 cites W2153103884 @default.
• W2014451608 cites W2156224208 @default.
• W2014451608 cites W2166190453 @default.
• W2014451608 cites W4686226 @default.
• W2014451608 doi "https://doi.org/10.3389/fpls.2014.00140" @default.
• W2014451608 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4006058" @default.
• W2014451608 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24795734" @default.
• W2014451608 hasPublicationYear "2014" @default.
• W2014451608 type Work @default.
• W2014451608 sameAs 2014451608 @default.
• W2014451608 citedByCount "45" @default.
• W2014451608 countsByYear W20144516082014 @default.
• W2014451608 countsByYear W20144516082015 @default.
• W2014451608 countsByYear W20144516082016 @default.
• W2014451608 countsByYear W20144516082017 @default.
• W2014451608 countsByYear W20144516082018 @default.
• W2014451608 countsByYear W20144516082019 @default.
• W2014451608 countsByYear W20144516082020 @default.
• W2014451608 countsByYear W20144516082021 @default.
• W2014451608 countsByYear W20144516082022 @default.
• W2014451608 countsByYear W20144516082023 @default.
• W2014451608 crossrefType "journal-article" @default.
• W2014451608 hasAuthorship W2014451608A5003362374 @default.
• W2014451608 hasAuthorship W2014451608A5011671405 @default.
• W2014451608 hasAuthorship W2014451608A5016900158 @default.
• W2014451608 hasAuthorship W2014451608A5023119806 @default.
• W2014451608 hasAuthorship W2014451608A5026839832 @default.
• W2014451608 hasAuthorship W2014451608A5037813372 @default.
• W2014451608 hasAuthorship W2014451608A5044369215 @default.
• W2014451608 hasAuthorship W2014451608A5064805550 @default.
• W2014451608 hasAuthorship W2014451608A5066038343 @default.
• W2014451608 hasAuthorship W2014451608A5086504077 @default.
• W2014451608 hasBestOaLocation W20144516081 @default.
• W2014451608 hasConcept C120665830 @default.
• W2014451608 hasConcept C121332964 @default.
• W2014451608 hasConcept C12554922 @default.
• W2014451608 hasConcept C136229726 @default.
• W2014451608 hasConcept C147080431 @default.
• W2014451608 hasConcept C1491633281 @default.
• W2014451608 hasConcept C15526883 @default.
• W2014451608 hasConcept C169274487 @default.
• W2014451608 hasConcept C192562407 @default.
• W2014451608 hasConcept C2779178360 @default.
• W2014451608 hasConcept C47785574 @default.
• W2014451608 hasConcept C55493867 @default.
• W2014451608 hasConcept C57477423 @default.
• W2014451608 hasConcept C67649825 @default.
• W2014451608 hasConcept C71924100 @default.
• W2014451608 hasConcept C86803240 @default.
• W2014451608 hasConcept C91881484 @default.
• W2014451608 hasConcept C95444343 @default.
• W2014451608 hasConceptScore W2014451608C120665830 @default.
• W2014451608 hasConceptScore W2014451608C121332964 @default.
• W2014451608 hasConceptScore W2014451608C12554922 @default.
• W2014451608 hasConceptScore W2014451608C136229726 @default.
• W2014451608 hasConceptScore W2014451608C147080431 @default.
• W2014451608 hasConceptScore W2014451608C1491633281 @default.
• W2014451608 hasConceptScore W2014451608C15526883 @default.
• W2014451608 hasConceptScore W2014451608C169274487 @default.
• W2014451608 hasConceptScore W2014451608C192562407 @default.
• W2014451608 hasConceptScore W2014451608C2779178360 @default.
• W2014451608 hasConceptScore W2014451608C47785574 @default.

• next