Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014470026> ?p ?o ?g. }
- W2014470026 endingPage "264" @default.
- W2014470026 startingPage "256" @default.
- W2014470026 abstract "The hemagglutinin from the seeds of Artocarpus hirsuta was purified to homogeneity by ion-exchange chromatography on DEAE-cellulose and CM-sephadex. The native protein of molecular mass 60,000 (gel filtration) is made up of two pairs of unidentical subunits, alpha and beta with molecular masses of 15,800 and 14,130. The lectin is basic in nature (pI 8.5) and a glycoprotein with neutral sugar content of 6.25%. Rabbit as well as human erythrocytes (A, B and O) are agglutinated by the lectin. The lectin activity is neither affected by Ca2+, Mg2+ or Mn2+ nor by EDTA. Methyl alpha-D-galactopyranoside, pNP-alpha-D-galactopyranoside and pNP-alpha-D-N-acetylgalactosamine are the best inhibitors of the lectin. 4-Methylumbelliferyl-alpha-galactopyranoside fluorescence was quenched on binding to A. hirsuta lectin. The sugar has two binding sites per tetramer of the lectin with a Ka of 3.5x105 M-1 at 25 degrees C. Chemical modification studies indicate that the net positive charge associated with epsilon-NH2 of lysine residues and the phenyl ring of tyrosine are essential for the sugar binding activity of A. hirsuta lectin." @default.
- W2014470026 created "2016-06-24" @default.
- W2014470026 creator A5027232650 @default.
- W2014470026 creator A5071679092 @default.
- W2014470026 creator A5085069846 @default.
- W2014470026 date "1998-07-01" @default.
- W2014470026 modified "2023-10-15" @default.
- W2014470026 title "α-Galactoside binding lectin from Artocarpus hirsuta: characterization of the sugar specificity and binding site" @default.
- W2014470026 cites W105515906 @default.
- W2014470026 cites W1575068560 @default.
- W2014470026 cites W1596232811 @default.
- W2014470026 cites W1600003190 @default.
- W2014470026 cites W1775749144 @default.
- W2014470026 cites W1968834637 @default.
- W2014470026 cites W1971653659 @default.
- W2014470026 cites W1977093640 @default.
- W2014470026 cites W1978761993 @default.
- W2014470026 cites W1980529383 @default.
- W2014470026 cites W1983649348 @default.
- W2014470026 cites W1983985826 @default.
- W2014470026 cites W1989932817 @default.
- W2014470026 cites W2003687284 @default.
- W2014470026 cites W2006370393 @default.
- W2014470026 cites W2008958194 @default.
- W2014470026 cites W2023576370 @default.
- W2014470026 cites W2024389470 @default.
- W2014470026 cites W2049491342 @default.
- W2014470026 cites W2053994489 @default.
- W2014470026 cites W2061686052 @default.
- W2014470026 cites W2072880784 @default.
- W2014470026 cites W2083001650 @default.
- W2014470026 cites W2088664516 @default.
- W2014470026 cites W2092974083 @default.
- W2014470026 cites W2100837269 @default.
- W2014470026 cites W2120657153 @default.
- W2014470026 cites W2137302959 @default.
- W2014470026 cites W2165865562 @default.
- W2014470026 cites W2209930309 @default.
- W2014470026 cites W2217145073 @default.
- W2014470026 cites W2320888265 @default.
- W2014470026 cites W2396461177 @default.
- W2014470026 doi "https://doi.org/10.1016/s0304-4165(98)00034-8" @default.
- W2014470026 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9685671" @default.
- W2014470026 hasPublicationYear "1998" @default.
- W2014470026 type Work @default.
- W2014470026 sameAs 2014470026 @default.
- W2014470026 citedByCount "26" @default.
- W2014470026 countsByYear W20144700262013 @default.
- W2014470026 countsByYear W20144700262014 @default.
- W2014470026 countsByYear W20144700262015 @default.
- W2014470026 countsByYear W20144700262016 @default.
- W2014470026 countsByYear W20144700262018 @default.
- W2014470026 countsByYear W20144700262019 @default.
- W2014470026 countsByYear W20144700262022 @default.
- W2014470026 crossrefType "journal-article" @default.
- W2014470026 hasAuthorship W2014470026A5027232650 @default.
- W2014470026 hasAuthorship W2014470026A5071679092 @default.
- W2014470026 hasAuthorship W2014470026A5085069846 @default.
- W2014470026 hasConcept C107824862 @default.
- W2014470026 hasConcept C108625454 @default.
- W2014470026 hasConcept C163994747 @default.
- W2014470026 hasConcept C181199279 @default.
- W2014470026 hasConcept C185592680 @default.
- W2014470026 hasConcept C193363816 @default.
- W2014470026 hasConcept C19869320 @default.
- W2014470026 hasConcept C202751555 @default.
- W2014470026 hasConcept C2777108408 @default.
- W2014470026 hasConcept C2777674797 @default.
- W2014470026 hasConcept C2778886173 @default.
- W2014470026 hasConcept C2779155592 @default.
- W2014470026 hasConcept C2780758401 @default.
- W2014470026 hasConcept C55493867 @default.
- W2014470026 hasConcept C58121356 @default.
- W2014470026 hasConcept C59822182 @default.
- W2014470026 hasConcept C71240020 @default.
- W2014470026 hasConcept C86803240 @default.
- W2014470026 hasConceptScore W2014470026C107824862 @default.
- W2014470026 hasConceptScore W2014470026C108625454 @default.
- W2014470026 hasConceptScore W2014470026C163994747 @default.
- W2014470026 hasConceptScore W2014470026C181199279 @default.
- W2014470026 hasConceptScore W2014470026C185592680 @default.
- W2014470026 hasConceptScore W2014470026C193363816 @default.
- W2014470026 hasConceptScore W2014470026C19869320 @default.
- W2014470026 hasConceptScore W2014470026C202751555 @default.
- W2014470026 hasConceptScore W2014470026C2777108408 @default.
- W2014470026 hasConceptScore W2014470026C2777674797 @default.
- W2014470026 hasConceptScore W2014470026C2778886173 @default.
- W2014470026 hasConceptScore W2014470026C2779155592 @default.
- W2014470026 hasConceptScore W2014470026C2780758401 @default.
- W2014470026 hasConceptScore W2014470026C55493867 @default.
- W2014470026 hasConceptScore W2014470026C58121356 @default.
- W2014470026 hasConceptScore W2014470026C59822182 @default.
- W2014470026 hasConceptScore W2014470026C71240020 @default.
- W2014470026 hasConceptScore W2014470026C86803240 @default.
- W2014470026 hasIssue "2" @default.
- W2014470026 hasLocation W20144700261 @default.
- W2014470026 hasLocation W20144700262 @default.
- W2014470026 hasOpenAccess W2014470026 @default.