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- W2014489511 abstract "Transgenic plants of Stylosanthes humilis cv. Paterson (Townsville Stylo) were regenerated from kanamycin-resistant callus obtained from leaf sections inoculated with Agrobacterium tumefaciens carrying the non-oncogenic Ti plasmid vector pGV3850: 1103neo (Hain et al., Mol. Gen. Genet., 199 (1985) 161–168). This vector contains the chimaeric gene coding for Tn5 neomycin phosphotransferase II (NPT) and the nopaline synthase gene, as markers. Both nopaline and NPT were detected in cell extracts of the transgenic plants. The presence of the Tn5 NPT coding sequences were demonstrated in DNA isolated from the transgenic plants by hybridisation. The inheritance of the gene for nopaline synthase was followed through 2 selfed generations and segregations consistent with Mendelian inheritance were observed in the R2 generation." @default.
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- W2014489511 date "1988-01-01" @default.
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- W2014489511 title "Transgenic plants of the tropical pasture legume stylosanthes humilis" @default.
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- W2014489511 doi "https://doi.org/10.1016/0168-9452(88)90042-8" @default.
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