Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014532072> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2014532072 endingPage "219" @default.
- W2014532072 startingPage "210" @default.
- W2014532072 abstract "Mono- and dinucleosomes preferentially cleaved from mouse myeloma chromatin by very mild micrococcal nuclease digestion at 0 degree C are soluble and are released from nuclei under near-physiological conditions in which normal nucleosomes containing Hl are insoluble. These nucleosomes are highly enriched in RNA, high-mobility-group proteins and a unique subset of other non-histone proteins. They are nearly devoid of histone Hl and contain DNA significantly less methylated than whole myeloma DNA, indicating that they comprise a subset of genomic sequences. Previously we have shown that this fraction is enriched in transcribed DNA sequences. Non-histone proteins that co-sedimented with readily solubilized nucleosomes included many of the most basic, low-to-moderate molecular weight chromosomal proteins. Many of these proteins were also preferentially acetylated in vivo. The residual, pelleted chromatin was highly enriched in high molecular weight proteins (greater than 60 000), and very depleted in medium molecular weight proteins. Readily solubilized nucleoproteins sedimenting like mononucleosomes were partly resolved by electrophoresis, under non-denaturing conditions, into several subfractions differing significantly in non-histone protein contents. Methods described here should be useful for identifying and isolating non-histone proteins bound to nucleosomes and other chromatin regions that are structurally and functionally unique." @default.
- W2014532072 created "2016-06-24" @default.
- W2014532072 creator A5007239518 @default.
- W2014532072 creator A5018803067 @default.
- W2014532072 date "1984-06-01" @default.
- W2014532072 modified "2023-09-27" @default.
- W2014532072 title "Non-histone proteins of soluble nucleoproteins released from mouse myeloma nuclei by mild micrococcal nuclease digestion" @default.
- W2014532072 cites W1421091744 @default.
- W2014532072 cites W1484760080 @default.
- W2014532072 cites W172832119 @default.
- W2014532072 cites W1965629295 @default.
- W2014532072 cites W1966872438 @default.
- W2014532072 cites W1975417880 @default.
- W2014532072 cites W1977105702 @default.
- W2014532072 cites W2026444338 @default.
- W2014532072 cites W2041086054 @default.
- W2014532072 cites W2044936649 @default.
- W2014532072 cites W2078825043 @default.
- W2014532072 cites W2092727047 @default.
- W2014532072 cites W4235025410 @default.
- W2014532072 cites W4254147892 @default.
- W2014532072 doi "https://doi.org/10.1016/0167-4781(84)90026-5" @default.
- W2014532072 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6722166" @default.
- W2014532072 hasPublicationYear "1984" @default.
- W2014532072 type Work @default.
- W2014532072 sameAs 2014532072 @default.
- W2014532072 citedByCount "6" @default.
- W2014532072 crossrefType "journal-article" @default.
- W2014532072 hasAuthorship W2014532072A5007239518 @default.
- W2014532072 hasAuthorship W2014532072A5018803067 @default.
- W2014532072 hasConcept C101152365 @default.
- W2014532072 hasConcept C139024627 @default.
- W2014532072 hasConcept C153911025 @default.
- W2014532072 hasConcept C185592680 @default.
- W2014532072 hasConcept C552990157 @default.
- W2014532072 hasConcept C55493867 @default.
- W2014532072 hasConcept C60325843 @default.
- W2014532072 hasConcept C64927066 @default.
- W2014532072 hasConcept C83640560 @default.
- W2014532072 hasConcept C84772758 @default.
- W2014532072 hasConcept C86803240 @default.
- W2014532072 hasConcept C87026931 @default.
- W2014532072 hasConceptScore W2014532072C101152365 @default.
- W2014532072 hasConceptScore W2014532072C139024627 @default.
- W2014532072 hasConceptScore W2014532072C153911025 @default.
- W2014532072 hasConceptScore W2014532072C185592680 @default.
- W2014532072 hasConceptScore W2014532072C552990157 @default.
- W2014532072 hasConceptScore W2014532072C55493867 @default.
- W2014532072 hasConceptScore W2014532072C60325843 @default.
- W2014532072 hasConceptScore W2014532072C64927066 @default.
- W2014532072 hasConceptScore W2014532072C83640560 @default.
- W2014532072 hasConceptScore W2014532072C84772758 @default.
- W2014532072 hasConceptScore W2014532072C86803240 @default.
- W2014532072 hasConceptScore W2014532072C87026931 @default.
- W2014532072 hasIssue "2" @default.
- W2014532072 hasLocation W20145320721 @default.
- W2014532072 hasLocation W20145320722 @default.
- W2014532072 hasOpenAccess W2014532072 @default.
- W2014532072 hasPrimaryLocation W20145320721 @default.
- W2014532072 hasRelatedWork W1796206844 @default.
- W2014532072 hasRelatedWork W1980892586 @default.
- W2014532072 hasRelatedWork W2010124452 @default.
- W2014532072 hasRelatedWork W2013914887 @default.
- W2014532072 hasRelatedWork W2014532072 @default.
- W2014532072 hasRelatedWork W2015776389 @default.
- W2014532072 hasRelatedWork W2016891566 @default.
- W2014532072 hasRelatedWork W2022534851 @default.
- W2014532072 hasRelatedWork W2120207521 @default.
- W2014532072 hasRelatedWork W4255074859 @default.
- W2014532072 hasVolume "782" @default.
- W2014532072 isParatext "false" @default.
- W2014532072 isRetracted "false" @default.
- W2014532072 magId "2014532072" @default.
- W2014532072 workType "article" @default.