Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014545539> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2014545539 abstract "The F1 complex of the ATP synthase of Streptomyces lividans was isolated and purified. The procedure involved the solubilization of F1 from membranes with buffer of low ionic strength in the presence of EDTA, ion-exchange chromatography and gel filtration. The purified F1 complex from S. lividans (SLF1) consists of five subunits alpha, beta, gamma, delta and epsilon with molecular masses of 58,000, 50,000, 36,000, 28,000 and 13,000, respectively and exhibits immunological cross-reactivity with the F1 portion purified from Escherichia coli (ECF1). The enzymatic properties of SLF1 were determined by the use of microtiter-plate-based assay and compared with data obtained for ECF1. ATPase activity of SLF1 (specific activity: 20-30 U/mg) was only observed in the presence of high concentrations of Ca2+ (10mM). Stimulation of the ATPase activity by Mg2+ was not detectable; quite to the contrary, Mg2+ inhibited the Ca(2+)-stimulated activity of SLF1. SLF1 was re-bound to F1-stripped membranes of S. lividans, but not to F1-stripped membrane vesicles of E. coli. In contrast, ECF1 could be cross-reconstituted with F1-stripped membranes of S. lividans; however, a structural but not a functional reconstitution of the hybrid F1Fo complex was observed." @default.
- W2014545539 created "2016-06-24" @default.
- W2014545539 creator A5025003953 @default.
- W2014545539 creator A5061209530 @default.
- W2014545539 creator A5091864825 @default.
- W2014545539 date "1991-12-01" @default.
- W2014545539 modified "2023-09-27" @default.
- W2014545539 title "Purification and characterization of the F1 portion of the ATP synthase (F1Fo) of Streptomyces lividans" @default.
- W2014545539 cites W1509156825 @default.
- W2014545539 cites W1513105997 @default.
- W2014545539 cites W1528950153 @default.
- W2014545539 cites W1578993307 @default.
- W2014545539 cites W1584592476 @default.
- W2014545539 cites W1595947986 @default.
- W2014545539 cites W174734255 @default.
- W2014545539 cites W1893428451 @default.
- W2014545539 cites W1919279082 @default.
- W2014545539 cites W1971573752 @default.
- W2014545539 cites W1975085160 @default.
- W2014545539 cites W1982204475 @default.
- W2014545539 cites W1990194240 @default.
- W2014545539 cites W2004595816 @default.
- W2014545539 cites W2010978494 @default.
- W2014545539 cites W2036053507 @default.
- W2014545539 cites W2041160133 @default.
- W2014545539 cites W2042846004 @default.
- W2014545539 cites W2045952010 @default.
- W2014545539 cites W2047459324 @default.
- W2014545539 cites W2052794591 @default.
- W2014545539 cites W2070635781 @default.
- W2014545539 cites W2082539152 @default.
- W2014545539 cites W2085418908 @default.
- W2014545539 cites W2088782754 @default.
- W2014545539 cites W2100837269 @default.
- W2014545539 cites W2115055430 @default.
- W2014545539 cites W2260696456 @default.
- W2014545539 cites W2316816840 @default.
- W2014545539 cites W2893905744 @default.
- W2014545539 cites W4239264452 @default.
- W2014545539 cites W4252295514 @default.
- W2014545539 cites W4253668170 @default.
- W2014545539 cites W89886074 @default.
- W2014545539 cites W940824992 @default.
- W2014545539 doi "https://doi.org/10.1111/j.1432-1033.1991.tb16505.x" @default.
- W2014545539 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1837270" @default.
- W2014545539 hasPublicationYear "1991" @default.
- W2014545539 type Work @default.
- W2014545539 sameAs 2014545539 @default.
- W2014545539 citedByCount "11" @default.
- W2014545539 countsByYear W20145455392014 @default.
- W2014545539 countsByYear W20145455392015 @default.
- W2014545539 countsByYear W20145455392016 @default.
- W2014545539 countsByYear W20145455392018 @default.
- W2014545539 crossrefType "journal-article" @default.
- W2014545539 hasAuthorship W2014545539A5025003953 @default.
- W2014545539 hasAuthorship W2014545539A5061209530 @default.
- W2014545539 hasAuthorship W2014545539A5091864825 @default.
- W2014545539 hasBestOaLocation W20145455391 @default.
- W2014545539 hasConcept C104317684 @default.
- W2014545539 hasConcept C112243037 @default.
- W2014545539 hasConcept C130316041 @default.
- W2014545539 hasConcept C181199279 @default.
- W2014545539 hasConcept C185592680 @default.
- W2014545539 hasConcept C193363816 @default.
- W2014545539 hasConcept C23265538 @default.
- W2014545539 hasConcept C41625074 @default.
- W2014545539 hasConcept C43617362 @default.
- W2014545539 hasConcept C547475151 @default.
- W2014545539 hasConcept C55493867 @default.
- W2014545539 hasConcept C86803240 @default.
- W2014545539 hasConceptScore W2014545539C104317684 @default.
- W2014545539 hasConceptScore W2014545539C112243037 @default.
- W2014545539 hasConceptScore W2014545539C130316041 @default.
- W2014545539 hasConceptScore W2014545539C181199279 @default.
- W2014545539 hasConceptScore W2014545539C185592680 @default.
- W2014545539 hasConceptScore W2014545539C193363816 @default.
- W2014545539 hasConceptScore W2014545539C23265538 @default.
- W2014545539 hasConceptScore W2014545539C41625074 @default.
- W2014545539 hasConceptScore W2014545539C43617362 @default.
- W2014545539 hasConceptScore W2014545539C547475151 @default.
- W2014545539 hasConceptScore W2014545539C55493867 @default.
- W2014545539 hasConceptScore W2014545539C86803240 @default.
- W2014545539 hasLocation W20145455391 @default.
- W2014545539 hasLocation W20145455392 @default.
- W2014545539 hasOpenAccess W2014545539 @default.
- W2014545539 hasPrimaryLocation W20145455391 @default.
- W2014545539 hasRelatedWork W1465188717 @default.
- W2014545539 hasRelatedWork W1771266178 @default.
- W2014545539 hasRelatedWork W1972665833 @default.
- W2014545539 hasRelatedWork W1978062153 @default.
- W2014545539 hasRelatedWork W2037443437 @default.
- W2014545539 hasRelatedWork W2050917233 @default.
- W2014545539 hasRelatedWork W2119806952 @default.
- W2014545539 hasRelatedWork W2150227713 @default.
- W2014545539 hasRelatedWork W2221554211 @default.
- W2014545539 hasRelatedWork W2375865423 @default.
- W2014545539 isParatext "false" @default.
- W2014545539 isRetracted "false" @default.
- W2014545539 magId "2014545539" @default.
- W2014545539 workType "article" @default.