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• W2014557018 abstract "Hydrogen in organic tissue resides in a complex mixture of molecular contexts. Some hydrogen, called non-exchangeable (Hnon), is strongly bound, and its isotopic ratio is fixed when the tissue is synthesized. Other pools of hydrogen, called exchangeable hydrogen (Hex), constantly exchange with ambient water vapor. The measurement of the δ2Hnon in organic tissues such as hair or feather therefore requires an analytical process that accounts for exchangeable hydrogen. In this study, swan feather and sheep wool keratin were used to test the effects of sample drying and capsule closure on the measurement of δ2Hnon values, and the rate of back-reaction with ambient water vapor. Homogenous feather or wool keratins were also calibrated at room temperature for use as control standards to correct for the effects of exchangeable hydrogen on feathers. Total δ2H values of both feather and wool samples showed large changes throughout the first ∼6 h of drying. Desiccant plus low vacuum seems to be more effective than room temperature vacuum pumping for drying samples. The degree of capsule closure affects exchangeable hydrogen equilibration and drying, with closed capsules responding more slowly. Using one control keratin standard to correct for the δ2Hex value for a batch of samples leads to internally consistent δ2Hnon values for other calibrated keratins run as unknowns. When placed in the context of other recent improvements in the measurement of keratin δ2Hnon values, we make recommendations for sample handing, data calibration and the reporting of results." @default.
• W2014557018 created "2016-06-24" @default.
• W2014557018 creator A5040717339 @default.
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• W2014557018 date "2014-10-31" @default.
• W2014557018 modified "2023-09-27" @default.
• W2014557018 title "Hydrogen isotope measurement of bird feather keratin, one laboratory's response to evolving methodologies" @default.
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• W2014557018 doi "https://doi.org/10.1080/10256016.2015.969256" @default.
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