FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014564496> ?p ?o ?g. }

• W2014564496 endingPage "1177" @default.
• W2014564496 startingPage "1168" @default.
• W2014564496 abstract "The complex amiB-mutL-miaA-hfq-hflX-hflK-hflC superoperon of E coli contains important genes for several fundamental cellular processes, including cell-wall hydrolysis (amiB), DNA repair (mutL), tRNA modification (miaA) and proteolysis (hflX-hflK-hflC). We report here the transcriptional pattern and possible posttranscriptional regulation of mutL, miaA and hfq genes of this superoperon. RNase protection analysis of mRNA transcribed from the bacterial chromosome demonstrated that there is co-transcription of mutl and miaA. In addition, two internal promoters, PmiaA and P1hfq were identified and mapped to 201 and 837 nucleotides upstream from the respective translation start sites. PmiaA contains poor matches to the −10 and −35 regions of the sigma-70 RNA polymerase concensus sequences, but it contains multiple potential Fis-binding sites and an upstream AT-rich region with poly(A) sequences. The basic arrangement of Fis-binding sites followed by an AT rich region is shared with promoters for rRNA operons and some of the tRNA and tRNA modification genes. As part of an initial study of mutL and miaA regulation, we measured transcript amounts in isogenic rne, rnc and rne rnc double mutants which are deficient in RNase E, RNase III or both. The amounts of steady state level mutL-miaA cotranscript, PmiaA transcript and Plhfq transcript increased eight-, nine- and three-fold respectively in an rne3071 mutant when compared to the rne+ parent. In contrast, amounts of the three transcripts were the same in an rnc105 mutant and its rnc+ parent. These results indicate that mutL, miaA, and hfq expression could be regulated by multiple mechanisms, including degree of cotranscription from upstream genes, modulation of internal promoter strength, and by RNase E activity. A model is presented for RNase E-mediated posttranscriptional regulation that may coordinated mutL expression with replication and miaA with tRNA amounts under different growth conditions, especially during nutrient upshifts." @default.
• W2014564496 created "2016-06-24" @default.
• W2014564496 creator A5006090738 @default.
• W2014564496 creator A5071662473 @default.
• W2014564496 date "1994-01-01" @default.
• W2014564496 modified "2023-10-01" @default.
• W2014564496 title "Transcriptional patterns of the mutL-miaA superoperon of Escherichia coli K-12 suggest a model for posttranscriptional regulation" @default.
• W2014564496 cites W141649614 @default.
• W2014564496 cites W1487552800 @default.
• W2014564496 cites W1519211795 @default.
• W2014564496 cites W1531182073 @default.
• W2014564496 cites W1541196086 @default.
• W2014564496 cites W1571932130 @default.
• W2014564496 cites W1587743369 @default.
• W2014564496 cites W1598109190 @default.
• W2014564496 cites W162291407 @default.
• W2014564496 cites W1665716161 @default.
• W2014564496 cites W1673573808 @default.
• W2014564496 cites W1691962367 @default.
• W2014564496 cites W1699731869 @default.
• W2014564496 cites W1773990555 @default.
• W2014564496 cites W1832605737 @default.
• W2014564496 cites W1917073860 @default.
• W2014564496 cites W1927691854 @default.
• W2014564496 cites W1945701981 @default.
• W2014564496 cites W1956228355 @default.
• W2014564496 cites W1972630690 @default.
• W2014564496 cites W1981112371 @default.
• W2014564496 cites W1981969047 @default.
• W2014564496 cites W1989781175 @default.
• W2014564496 cites W2014571977 @default.
• W2014564496 cites W2016245855 @default.
• W2014564496 cites W2028011256 @default.
• W2014564496 cites W2038818905 @default.
• W2014564496 cites W2047221174 @default.
• W2014564496 cites W2049737239 @default.
• W2014564496 cites W2052200921 @default.
• W2014564496 cites W2053827853 @default.
• W2014564496 cites W2055145020 @default.
• W2014564496 cites W2072989645 @default.
• W2014564496 cites W2077685286 @default.
• W2014564496 cites W2088191152 @default.
• W2014564496 cites W2096641315 @default.
• W2014564496 cites W2099105015 @default.
• W2014564496 cites W2110460574 @default.
• W2014564496 cites W2114025420 @default.
• W2014564496 cites W2140460966 @default.
• W2014564496 cites W2148525353 @default.
• W2014564496 cites W2148922163 @default.
• W2014564496 cites W2161203597 @default.
• W2014564496 cites W2168149109 @default.
• W2014564496 cites W2174136357 @default.
• W2014564496 doi "https://doi.org/10.1016/0300-9084(94)90046-9" @default.
• W2014564496 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7748952" @default.
• W2014564496 hasPublicationYear "1994" @default.
• W2014564496 type Work @default.
• W2014564496 sameAs 2014564496 @default.
• W2014564496 citedByCount "32" @default.
• W2014564496 countsByYear W20145644962012 @default.
• W2014564496 countsByYear W20145644962013 @default.
• W2014564496 countsByYear W20145644962014 @default.
• W2014564496 countsByYear W20145644962015 @default.
• W2014564496 countsByYear W20145644962016 @default.
• W2014564496 countsByYear W20145644962017 @default.
• W2014564496 countsByYear W20145644962018 @default.
• W2014564496 countsByYear W20145644962020 @default.
• W2014564496 countsByYear W20145644962021 @default.
• W2014564496 countsByYear W20145644962022 @default.
• W2014564496 crossrefType "journal-article" @default.
• W2014564496 hasAuthorship W2014564496A5006090738 @default.
• W2014564496 hasAuthorship W2014564496A5071662473 @default.
• W2014564496 hasConcept C104317684 @default.
• W2014564496 hasConcept C10879258 @default.
• W2014564496 hasConcept C138885662 @default.
• W2014564496 hasConcept C143065580 @default.
• W2014564496 hasConcept C153911025 @default.
• W2014564496 hasConcept C153957851 @default.
• W2014564496 hasConcept C179926584 @default.
• W2014564496 hasConcept C203075996 @default.
• W2014564496 hasConcept C2776449523 @default.
• W2014564496 hasConcept C41895202 @default.
• W2014564496 hasConcept C54355233 @default.
• W2014564496 hasConcept C67705224 @default.
• W2014564496 hasConcept C70916841 @default.
• W2014564496 hasConcept C86803240 @default.
• W2014564496 hasConceptScore W2014564496C104317684 @default.
• W2014564496 hasConceptScore W2014564496C10879258 @default.
• W2014564496 hasConceptScore W2014564496C138885662 @default.
• W2014564496 hasConceptScore W2014564496C143065580 @default.
• W2014564496 hasConceptScore W2014564496C153911025 @default.
• W2014564496 hasConceptScore W2014564496C153957851 @default.
• W2014564496 hasConceptScore W2014564496C179926584 @default.
• W2014564496 hasConceptScore W2014564496C203075996 @default.
• W2014564496 hasConceptScore W2014564496C2776449523 @default.
• W2014564496 hasConceptScore W2014564496C41895202 @default.
• W2014564496 hasConceptScore W2014564496C54355233 @default.
• W2014564496 hasConceptScore W2014564496C67705224 @default.
• W2014564496 hasConceptScore W2014564496C70916841 @default.

• next