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- W2014590398 abstract "We present a spectrally resolved confocal imaging approach to qualitatively asses the overall uptake and the penetrationdepth of fluorescent dyes into biological tissue. We use a confocal microscope with a spectral resolution of 5 nm tomeasure porcine skin tissue after performing a Franz-Diffusion experiment with a submicron emulsion enriched with thefluorescent dye Nile Red. The evaluation uses linear unmixing of the dye and the tissue autofluorescence spectra. Theresults are combined with a manual segmentation of the skin's epidermis and dermis layers to assess the penetrationbehavior additionally to the overall uptake. The diffusion experiments, performed for 3h and 24h, show a 3-foldincreased dye uptake in the epidermis and dermis for the 24h samples. As the method is based on spectral information itdoes not face the problem of superimposed dye and tissue spectra and therefore is more precise compared to intensitybased evaluation methods." @default.
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- W2014590398 date "2012-02-09" @default.
- W2014590398 modified "2023-09-23" @default.
- W2014590398 title "Spectrally resolved visualization of fluorescent dyes permeating into skin" @default.
- W2014590398 doi "https://doi.org/10.1117/12.907815" @default.
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