FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014604180> ?p ?o ?g. }

• W2014604180 endingPage "596" @default.
• W2014604180 startingPage "585" @default.
• W2014604180 abstract "As model compounds for Ni(II)-binding heparin-like compounds isolated from human kidneys (Templeton, D.M. & Sarkar, B. (1985) Biochem. J. 230 35-42.), we investigated two disaccharides--4-O-(2-O-sulfo-alpha-L-idopyranosyluronic acid)-2,5-anhydro- D-mannitol, disodium salt (1a), and 4-O-(2-O-sulfo-alpha-L-idopyranosyluronic acid)-6-O- sulfo-2,5-anhydro-D-mannitol, trisodium salt (1b)--that were isolated from heparin after nitrous acid hydrolysis and reduction. The monosulfate (1a) was active whereas the disulfate (1b) was inactive in a high-performance liquid chromatography (HPLC) binding assay with the tracer ions 63Ni(II) 54Mn(II), 65Zn(II), and 109Cd(II). This result is in accord with the isolation of two 67Cu(II) and 63Ni(II) binding fractions from a complete pool of nitrous-acid-derived heparin disaccharides using sulfate gradients and a MonoQ anion exchange column on an FPLC system. One was identified as compound (1a) and the other as a tetrasulfated trisaccharide by high resolution FAB-MS, NMR and HPLC-PAD. Similarly, two synthetic disaccharides-methyl, 2-O-sulfo-4-O-(alpha-L-idopyranosyluronic acid)-2-deoxy-2-sulfamide-alpha-D-glucosamine, trisodium salt [IdopA2S(alpha 1,4)GlcNS alpha Me, 2a], and 2-O-sulfo-4-O-(alpha-L-idopyranosyluronic acid)-2-deoxy-2-sulfamide-6-O-sulfo- alpha-D-glucosamine, tetrasodium salt [IdopA2S (alpha 1,4)GlcNS6S alpha Me, 2b]--were shown to bind tracer amounts of 63Ni and 67Cu using chromatographic assays. Subsequently, 1H NMR titrations of 1a, 1b, 2a, and 2b with Zn (OAc)2 were analyzed to yield 1:1 Zn(II)-binding constants of 472 +/- 59, 698 +/- 120, 8,758 +/- 2,237 and 20,100 +/- 5,598 M-1, respectively. The values for 2a and 2b suggest chelation. It is suggested that the idopyranosiduronic acid residue is the major metal binding site. NMR evidence for this hypothesis comes from marked 1H and 13C chemical shift changes to the iduronic acid resonances after addition of diamagnetic Zn(II) ions." @default.
• W2014604180 created "2016-06-24" @default.
• W2014604180 creator A5003596933 @default.
• W2014604180 creator A5059046284 @default.
• W2014604180 creator A5091378879 @default.
• W2014604180 date "1992-06-01" @default.
• W2014604180 modified "2023-09-25" @default.
• W2014604180 title "Heavy metal binding to heparin disaccharides. I. Iduronic acid is the main binding site" @default.
• W2014604180 cites W121528914 @default.
• W2014604180 cites W1562629022 @default.
• W2014604180 cites W1594150232 @default.
• W2014604180 cites W1883949913 @default.
• W2014604180 cites W190293211 @default.
• W2014604180 cites W1968321554 @default.
• W2014604180 cites W1968488748 @default.
• W2014604180 cites W1969324962 @default.
• W2014604180 cites W1979958766 @default.
• W2014604180 cites W1987317584 @default.
• W2014604180 cites W1992999462 @default.
• W2014604180 cites W1993916139 @default.
• W2014604180 cites W1997203824 @default.
• W2014604180 cites W2000959212 @default.
• W2014604180 cites W2003287774 @default.
• W2014604180 cites W2011238418 @default.
• W2014604180 cites W2016351542 @default.
• W2014604180 cites W2017626210 @default.
• W2014604180 cites W2024413530 @default.
• W2014604180 cites W2029690071 @default.
• W2014604180 cites W2031204274 @default.
• W2014604180 cites W2032255522 @default.
• W2014604180 cites W2036647212 @default.
• W2014604180 cites W2039401064 @default.
• W2014604180 cites W2041420763 @default.
• W2014604180 cites W2043469852 @default.
• W2014604180 cites W2064027616 @default.
• W2014604180 cites W2064528046 @default.
• W2014604180 cites W2071886853 @default.
• W2014604180 cites W2077341560 @default.
• W2014604180 cites W2127439444 @default.
• W2014604180 cites W2132082955 @default.
• W2014604180 cites W2228711123 @default.
• W2014604180 cites W2230659414 @default.
• W2014604180 cites W2271693112 @default.
• W2014604180 cites W2316324981 @default.
• W2014604180 doi "https://doi.org/10.1002/bip.360320603" @default.
• W2014604180 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1643264" @default.
• W2014604180 hasPublicationYear "1992" @default.
• W2014604180 type Work @default.
• W2014604180 sameAs 2014604180 @default.
• W2014604180 citedByCount "15" @default.
• W2014604180 countsByYear W20146041802014 @default.
• W2014604180 countsByYear W20146041802022 @default.
• W2014604180 crossrefType "journal-article" @default.
• W2014604180 hasAuthorship W2014604180A5003596933 @default.
• W2014604180 hasAuthorship W2014604180A5059046284 @default.
• W2014604180 hasAuthorship W2014604180A5091378879 @default.
• W2014604180 hasConcept C178790620 @default.
• W2014604180 hasConcept C185592680 @default.
• W2014604180 hasConcept C2776371256 @default.
• W2014604180 hasConcept C2777346074 @default.
• W2014604180 hasConcept C2778125326 @default.
• W2014604180 hasConcept C2778445172 @default.
• W2014604180 hasConcept C2780112151 @default.
• W2014604180 hasConcept C43617362 @default.
• W2014604180 hasConcept C71240020 @default.
• W2014604180 hasConcept C94412978 @default.
• W2014604180 hasConceptScore W2014604180C178790620 @default.
• W2014604180 hasConceptScore W2014604180C185592680 @default.
• W2014604180 hasConceptScore W2014604180C2776371256 @default.
• W2014604180 hasConceptScore W2014604180C2777346074 @default.
• W2014604180 hasConceptScore W2014604180C2778125326 @default.
• W2014604180 hasConceptScore W2014604180C2778445172 @default.
• W2014604180 hasConceptScore W2014604180C2780112151 @default.
• W2014604180 hasConceptScore W2014604180C43617362 @default.
• W2014604180 hasConceptScore W2014604180C71240020 @default.
• W2014604180 hasConceptScore W2014604180C94412978 @default.
• W2014604180 hasIssue "6" @default.
• W2014604180 hasLocation W20146041801 @default.
• W2014604180 hasLocation W20146041802 @default.
• W2014604180 hasOpenAccess W2014604180 @default.
• W2014604180 hasPrimaryLocation W20146041801 @default.
• W2014604180 hasRelatedWork W1975256406 @default.
• W2014604180 hasRelatedWork W1983694554 @default.
• W2014604180 hasRelatedWork W2091538365 @default.
• W2014604180 hasRelatedWork W2095581038 @default.
• W2014604180 hasRelatedWork W2139203759 @default.
• W2014604180 hasRelatedWork W2395908720 @default.
• W2014604180 hasRelatedWork W2952042668 @default.
• W2014604180 hasRelatedWork W2952740408 @default.
• W2014604180 hasRelatedWork W2998602881 @default.
• W2014604180 hasRelatedWork W4245703765 @default.
• W2014604180 hasVolume "32" @default.
• W2014604180 isParatext "false" @default.
• W2014604180 isRetracted "false" @default.
• W2014604180 magId "2014604180" @default.
• W2014604180 workType "article" @default.