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- W2014608137 abstract "The effect of various chemical, enzymatic, and heat treatments on the ability of basement membrane (BM) produced by cultured bovine corneal endothelial cells to support cell proliferation vs cell attachment has been analysed. Alkaline treatment (pH 13.9), which is known to cleave proteoglycans or glycopeptides at the O-glycosidic bond between the protein and carbohydrate moieties resulted in a 97% decrease in the ability of BM-coated dishes to support cell growth. Likewise, treatment with 4 M guanidine-HCl, which extracts up to 80% of the glycosaminoglycan, caused a 92% reduction in cell growth. Nitrous acid (HNO2) treatment, which degrades heparan sulphate and releases both sulphated disaccharide and non-sulphated oligosaccharides, also reduced cell growth by 90%. Treatment of the BM with specific protease, collagenase, or glycosidase did not appreciably decrease the ability of the BM to support cell growth. It is therefore doubtful that any of the substrates used for the enzymes tested in the present studies could play a direct role in cell proliferation. Alkali treatment of BM, which abolishes its ability to support cell growth, does not alter the cells' ability to attach to it. On the contrary, such treatment even improves it slightly. Conversely, heat treatment of BM (70 °C), which does not reduce the ability of the cells to grow on BM, strongly reduces their ability to attach to it. It is therefore likely that heat treatment, but not alkali treatment of BM preferentially affects attachment factors, but not growth-promoting factors, and that these factors are separate entities." @default.
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- W2014608137 title "Inactivation of a basement membrane component responsible for cell proliferation but not for cell attachment" @default.
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- W2014608137 doi "https://doi.org/10.1016/0014-4827(82)90149-5" @default.
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